Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,874,4610AG54.9% 13.9 / 67.8 51I175I (ATT→ATCcysDsulfate adenylyltransferase, subunit 2
Reads supporting (aligned to +/- strand):  ref base A (15/8);  new base G (16/12);  total (31/20)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGATCCCAGC  >  W3110S.gb/2874400‑2874516
                                                             |                                                       
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:185609/1‑63 (MQ=255)
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:906621/1‑63 (MQ=255)
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:2493113/1‑63 (MQ=255)
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:3327947/1‑63 (MQ=255)
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:2742417/1‑63 (MQ=255)
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:1501934/1‑63 (MQ=255)
gCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaa                                                        >  1:1494815/1‑63 (MQ=255)
   aGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTg                                             >  1:3301123/1‑71 (MQ=255)
    gATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGt                                            <  1:2157488/71‑1 (MQ=255)
    gATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGt                                            <  1:2442256/71‑1 (MQ=255)
    gATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGt                                            <  1:3212748/71‑1 (MQ=255)
    gATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGt                                            <  1:3673250/71‑1 (MQ=255)
    gATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGt                                            <  1:4042440/71‑1 (MQ=255)
     atatCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           <  1:236892/71‑1 (MQ=255)
      tatCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           <  1:968184/70‑1 (MQ=255)
       atCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGt                                         >  1:3202587/1‑71 (MQ=255)
       atCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGt                                         >  1:2166247/1‑71 (MQ=255)
       atCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGt                                         >  1:2783918/1‑71 (MQ=255)
       atCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGt                                         >  1:173034/1‑71 (MQ=255)
        tCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGtt                                        >  1:2864429/1‑71 (MQ=255)
          cTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTtg                                       >  1:780560/1‑70 (MQ=255)
          cTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTtg                                       >  1:1071656/1‑70 (MQ=255)
                  tCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGc                              <  1:4058474/71‑1 (MQ=255)
                  tCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGc                              <  1:2466376/71‑1 (MQ=255)
                  tCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGc                              <  1:3784801/71‑1 (MQ=255)
                    cAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGc                              <  1:2894126/69‑1 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:1277730/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:2291183/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:3303782/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:3762831/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:3870340/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:638310/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:3925064/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:3932901/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:1239897/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:738387/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:2746362/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:16874/1‑53 (MQ=255)
                        tAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAg                                          >  1:2320334/1‑53 (MQ=255)
                         agagagCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGc                              >  1:4123425/1‑64 (MQ=255)
                         agagagCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCggg                        >  1:3139933/1‑70 (MQ=255)
                                   aaGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGAttttt             >  1:1271387/1‑71 (MQ=255)
                                   aaGACGCGGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGAttttt             >  1:379111/1‑71 (MQ=255)
                                       cgcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGa         <  1:1392180/71‑1 (MQ=255)
                                       cgcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGa         <  1:1273773/71‑1 (MQ=255)
                                       cgcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGa         <  1:724111/71‑1 (MQ=255)
                                       cgcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGa         <  1:1683280/71‑1 (MQ=255)
                                       cgcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGa         <  1:179824/71‑1 (MQ=255)
                                        gcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           >  1:1202010/1‑36 (MQ=255)
                                        gcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           >  1:1197639/1‑36 (MQ=255)
                                        gcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           >  1:3352838/1‑36 (MQ=255)
                                        gcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           >  1:1467270/1‑36 (MQ=255)
                                        gcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           >  1:2761687/1‑36 (MQ=255)
                                        gcgGATGCTTTCGCCTTTGTTGATTTGCCCGTTGTa                                           >  1:2559159/1‑36 (MQ=255)
                                               cTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGATCCCAGc  <  1:3966993/70‑1 (MQ=255)
                                               cTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGATCCCAGc  <  1:3884780/70‑1 (MQ=255)
                                               cTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGATCCCAGc  <  1:733812/70‑1 (MQ=255)
                                               cTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGATCCCAGc  <  1:2150319/70‑1 (MQ=255)
                                                             |                                                       
GCCAGATATCCTGCTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGTAGTTGTGCCACAGCTCCGGGCGCTGATTTTTCGGATCCCAGC  >  W3110S.gb/2874400‑2874516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: