| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,885,984 | 0 | C | T | 53.6% | 8.7 / 99.6 | 69 | intergenic (‑109/+250) | ygcB/cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base C (17/15); new base T (23/14); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGA > W3110S.gb/2885928‑2886042 | tCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTa < 1:3122424/70‑1 (MQ=255)tCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTa < 1:3299920/70‑1 (MQ=255) gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga < 1:1627832/63‑1 (MQ=255) ctgctCATAAATATCGTATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:3961864/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAATAGACTAACATTc > 1:904790/1‑70 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:2184187/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:1021587/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:3232935/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:3007955/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:3300946/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:2738422/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:3335559/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:3561323/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:2237636/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:2179759/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:2041625/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:966422/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:1393537/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:639841/1‑61 (MQ=255) ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:624199/1‑61 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:99170/71‑1 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:3538124/71‑1 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:1683514/71‑1 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:2188497/71‑1 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCg > 1:3300591/1‑66 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:247981/1‑71 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:4007275/1‑71 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:677941/1‑71 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:3243433/1‑71 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:982114/1‑71 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:1229351/1‑71 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:488977/1‑71 (MQ=255) atatACACATGTCTTGCCTCCCTGCAAAGAAgaga > 1:772538/1‑35 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTc < 1:63443/69‑1 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTc < 1:3213522/69‑1 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTc < 1:2968986/69‑1 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGt > 1:2743928/1‑71 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGt > 1:2569785/1‑71 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGt > 1:3998488/1‑71 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGt > 1:1636486/1‑71 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGt > 1:3773277/1‑71 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGt > 1:1389389/1‑71 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 1:2847020/1‑68 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 1:1257055/1‑68 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 1:3383953/1‑68 (MQ=255) cTTGCCTCCCTGTAAAGAAGAGACTAACATTCGGATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAg > 1:1880031/1‑70 (MQ=255) cTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTg > 1:93743/1‑66 (MQ=255) cTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAg > 1:883920/1‑70 (MQ=255) cTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAg > 1:1615964/1‑70 (MQ=255) cTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAg > 1:1839258/1‑70 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACTCTGGCCTCAGGTTGATAGa < 1:127457/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:4081611/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:1124730/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:3136816/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:2546636/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:3092452/68‑1 (MQ=255) ccTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:2223481/67‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTTTTTGGGTTCGAACGCTGGCCTCa < 1:803145/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:421973/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:3969835/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:244220/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:1492944/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:3621954/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:3602500/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:128954/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:3566153/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:967778/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:2677797/51‑1 (MQ=255) tGCAAAGAAGAGACTAACATGCGAATTGTTTGGGTTCGAACGCTGGCCTCa < 1:821246/51‑1 (MQ=255) | TCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGA > W3110S.gb/2885928‑2886042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |