| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,971,401 | 0 | A | T | 58.8% | 30.2 / 62.3 | 51 | V373E (GTG→GAG) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (13/8); new base T (26/4); total (39/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTT > W3110S.gb/2971341‑2971466 | cAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:1091753/71‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTa > 1:2873595/1‑70 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTa > 1:3140667/1‑70 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:2204382/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:2340227/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:2843753/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:1720646/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:3038463/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:3371528/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:138487/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:794484/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:900635/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTAt > 1:1092200/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTAt > 1:3861666/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTAt > 1:3942333/1‑71 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTAt > 1:2582395/1‑71 (MQ=255) aGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCt < 1:807734/66‑1 (MQ=255) cgcCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCt < 1:2765725/64‑1 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:2114114/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:521076/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:243104/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:1405784/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:2753841/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:1886362/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:1140471/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:140597/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:3024423/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:3026780/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:3731483/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:3182353/1‑50 (MQ=255) aTTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg > 1:1561656/1‑50 (MQ=255) gCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGc > 1:1893124/1‑69 (MQ=255) aGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:3122483/71‑1 (MQ=255) aGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGACAGCGAGTa < 1:2420065/71‑1 (MQ=255) gcAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:1484212/44‑1 (MQ=255) gcAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCAt > 1:3738723/1‑53 (MQ=255) gcAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCAt > 1:24695/1‑53 (MQ=255) aGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCAt > 1:488325/1‑47 (MQ=255) atgGGCACGACCTGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGtt < 1:3606316/71‑1 (MQ=255) atgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGtt < 1:3084868/71‑1 (MQ=255) atgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGtt < 1:1747444/71‑1 (MQ=255) atgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGtt < 1:3966599/71‑1 (MQ=255) atgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGtt < 1:712535/71‑1 (MQ=255) atgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGtt < 1:1286236/71‑1 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:971615/1‑71 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:1231710/1‑71 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:89567/1‑71 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:3407346/1‑71 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:1602986/1‑71 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:1816348/1‑71 (MQ=255) tgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGttt > 1:1896677/1‑71 (MQ=255) | CAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTT > W3110S.gb/2971341‑2971466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |