Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,628,1040GA72.2% 52.7 / 25.0 36V161V (GTG→GTArhtBneutral amino‑acid efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/10);  new base A (14/12);  total (14/22)
Fisher's exact test for biased strand distribution p-value = 2.72e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCCGCAACTGATGCAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAGG  >  W3110S.gb/3628043‑3628171
                                                             |                                                                   
aGCCGCAACTGATGCAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            <  1:186710/71‑1 (MQ=255)
aGCCGCAACTGATGCAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            <  1:2399748/71‑1 (MQ=255)
aGCCGCAACTGATGCAGTATATCGTGCTCGGCGTCACCACTATTGAGGTCGATATTATTGTAATGATCGGt                                                            <  1:2086388/71‑1 (MQ=255)
              cAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCg                                                              <  1:1213297/55‑1 (MQ=255)
              cAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCg                                                              <  1:1672409/55‑1 (MQ=255)
              cAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCg                                                              <  1:3323346/55‑1 (MQ=255)
              cAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCg                                                              <  1:3272477/55‑1 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:415629/1‑55 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:4138893/1‑55 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:390088/1‑55 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:1576227/1‑55 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:3690556/1‑55 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:3657959/1‑55 (MQ=255)
                gTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGt                                                            >  1:2198720/1‑55 (MQ=255)
                   tatCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAAc                                         >  1:2959824/1‑71 (MQ=255)
                   tatCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAAc                                         >  1:2955174/1‑71 (MQ=255)
                   tatCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAAc                                         >  1:1426335/1‑71 (MQ=255)
                     tCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACg                                        >  1:847512/1‑70 (MQ=255)
                     tCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACg                                        >  1:1813189/1‑70 (MQ=255)
                              gCGTCACCACTATTGTGGTCGATATTATTGTAATGATCg                                                              <  1:4006459/39‑1 (MQ=255)
                                gTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATg                            <  1:854534/71‑1 (MQ=255)
                                gTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATg                            <  1:2189584/71‑1 (MQ=255)
                                gTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATg                            <  1:725879/71‑1 (MQ=255)
                                gTCACCACTATTGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATg                            <  1:4109033/71‑1 (MQ=255)
                                          ttGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGGTCAACGGATTGCTCTATGGATTAAAGGa                  >  1:2967536/1‑71 (MQ=255)
                                          ttGTGGTCGATATTATTGTAATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGa                  >  1:1191641/1‑71 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:1639653/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:717458/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:1363636/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:3996092/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:1461054/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:1698023/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:3476300/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:2059825/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:3208945/57‑1 (MQ=255)
                                                      ttattGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAg                    <  1:2674116/57‑1 (MQ=255)
                                                          tgtaATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAgg  >  1:2279483/1‑71 (MQ=255)
                                                          tgtaATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAgg  >  1:4063090/1‑71 (MQ=255)
                                                          tgtaATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAgg  >  1:4135502/1‑71 (MQ=255)
                                                          tgtaATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAgg  >  1:348977/1‑71 (MQ=255)
                                                          tgtaATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAg   >  1:2890485/1‑70 (MQ=255)
                                                             |                                                                   
AGCCGCAACTGATGCAGTATATCGTGCTCGGCGTCACCACTATTGTGGTCGATATTATTGTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAGG  >  W3110S.gb/3628043‑3628171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: