| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,861,751 | 0 | T | C | 73.3% | 160.8 / 61.2 | 90 | N423S (AAC→AGC) | lldP | L‑lactate permease |
| Reads supporting (aligned to +/- strand): ref base T (17/7); new base C (25/41); total (42/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCT > W3110S.gb/3861685‑3861810 | aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:3455500/71‑1 (MQ=255)aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:1238621/71‑1 (MQ=255)aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:2105984/71‑1 (MQ=255) agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:2951528/70‑1 (MQ=255) agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:3789907/70‑1 (MQ=255) agGTGAATGCATGCCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAAt > 1:3454277/1‑70 (MQ=255) agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAAt > 1:3137927/1‑70 (MQ=255) agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAAt > 1:215311/1‑70 (MQ=255) agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGAGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAAt > 1:511315/1‑70 (MQ=255) gTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATa > 1:2796709/1‑69 (MQ=255) gTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATa > 1:367025/1‑69 (MQ=255) gTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATa > 1:3357626/1‑69 (MQ=255) gTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATa > 1:965732/1‑69 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATaaac < 1:3759371/71‑2 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:173617/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:309222/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:346470/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:290410/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:818305/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:3122862/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:1956038/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:2085061/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:2696106/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:3277562/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:3816240/71‑1 (MQ=255) tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:80317/71‑1 (MQ=255) gAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAg < 1:1028786/70‑1 (MQ=255) aaTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGc > 1:1322342/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAAt < 1:1277983/71‑1 (MQ=255) aTGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAAt < 1:789040/70‑1 (MQ=255) tGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc > 1:790437/1‑71 (MQ=255) tGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc > 1:3988410/1‑71 (MQ=255) tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCa < 1:2566154/70‑1 (MQ=255) tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCa < 1:1797995/70‑1 (MQ=255) gCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCa < 1:3746093/61‑1 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg < 1:3045518/47‑1 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg < 1:432068/47‑1 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg < 1:2776249/71‑1 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg < 1:3321929/71‑1 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:2809041/1‑68 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:3314205/1‑68 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:188435/1‑68 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:421639/1‑68 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:2907996/1‑68 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:1764493/1‑68 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATg > 1:1864681/1‑66 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc < 1:3752222/40‑1 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc < 1:3782432/40‑1 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc > 1:1632691/1‑51 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:391056/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:505358/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:2990005/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:1079058/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:2907098/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:2295008/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:2388581/1‑69 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa > 1:2495296/1‑69 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGa < 1:1732701/55‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGa < 1:3805917/55‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:1064398/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:1213985/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:1500344/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:603397/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:545286/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:4018385/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:2277731/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:2687533/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:2755780/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:3672965/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:3064118/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:2898089/70‑1 (MQ=255) atgaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc < 1:286192/70‑1 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:2595419/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:853433/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:1535261/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:707394/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:1548530/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:3457190/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:1891817/1‑71 (MQ=255) gaCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:2767388/1‑71 (MQ=255) gTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCt < 1:1612305/71‑1 (MQ=255) gTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCt < 1:2937795/71‑1 (MQ=255) gTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCt < 1:3064606/71‑1 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:1792513/1‑65 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:3982029/1‑65 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:3437846/1‑65 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:2825335/1‑65 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:35279/1‑65 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:343438/1‑65 (MQ=255) gAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTc > 1:371419/1‑65 (MQ=255) | AAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCT > W3110S.gb/3861685‑3861810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |