Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	45,813	C→T	P3S (CCG→TCG)	yaaU →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	45,813	0	C	T	100.0%	175.5 / NA	55	P3S (CCG→TCG)	yaaU	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/22);  total (33/22)

TGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTG  >  W3110S.gb/45748‑45871                                                                  |                                                           tGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTc                                                        >  1:2280538/1‑70 (MQ=255) tGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTc                                                        >  1:1938448/1‑70 (MQ=255) tGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTc                                                        >  1:3634049/1‑70 (MQ=255) tGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTc                                                        >  1:571228/1‑70 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:326781/71‑1 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:1965445/71‑1 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:1583380/71‑1 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:1385771/71‑1 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:275560/71‑1 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:3369169/71‑1 (MQ=255)    tGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGa                                                    <  1:712466/71‑1 (MQ=255)        ggTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACt                                                >  1:2602257/1‑71 (MQ=255)        ggTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACt                                                >  1:3655834/1‑71 (MQ=255)        ggTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACt                                                >  1:1792393/1‑71 (MQ=255)        ggTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACt                                                >  1:2071795/1‑71 (MQ=255)              aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACg                                          <  1:3865716/71‑1 (MQ=255)              aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACg                                          <  1:445914/71‑1 (MQ=255)              aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACg                                          <  1:1552527/71‑1 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:3975620/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:1996041/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:1431817/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:2608266/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:1168396/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:3396863/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:3074006/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:3171111/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAtc                                       >  1:608175/1‑71 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAt                                        <  1:2368299/70‑1 (MQ=255)                 cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGAt                                        <  1:742443/70‑1 (MQ=255)                        ccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:1052106/71‑1 (MQ=255)                        ccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:593938/71‑1 (MQ=255)                        ccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:3469245/71‑1 (MQ=255)                        ccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:1000444/71‑1 (MQ=255)                        ccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:290652/71‑1 (MQ=255)                         cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACg                                          <  1:661291/60‑1 (MQ=255)                         cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:2828675/70‑1 (MQ=255)                         cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:2271681/70‑1 (MQ=255)                         cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:1296510/70‑1 (MQ=255)                         cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAAtt                                <  1:2169790/70‑1 (MQ=255)                           cACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACTATCTCAAATtctc                             >  1:2349750/1‑71 (MQ=255)                           cACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:1317861/1‑71 (MQ=255)                                  tctcATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTg                                             >  1:1107509/1‑48 (MQ=255)                                  tctcATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:1089002/1‑64 (MQ=255)                                        tGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTctcctc                          >  1:3184513/1‑61 (MQ=255)                                        tGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTctcctc                          >  1:661496/1‑61 (MQ=255)                                        tGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTctcctc                          >  1:3609305/1‑61 (MQ=255)                                        tGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTctcctc                          >  1:3241581/1‑61 (MQ=255)                                         gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:389593/1‑57 (MQ=255)                                         gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:623375/1‑57 (MQ=255)                                         gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:2362281/1‑57 (MQ=255)                                         gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:2887663/1‑57 (MQ=255)                                         gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:762102/1‑57 (MQ=255)                                         gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATtctc                             >  1:926226/1‑57 (MQ=255)                                                      aTAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCtgtg  >  1:4010373/1‑71 (MQ=255)                                                      aTAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCtgtg  >  1:818912/1‑71 (MQ=255)                                                                  |                                                           TGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTG  >  W3110S.gb/45748‑45871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑