| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 344,381 | G→A | intergenic (+166/‑247) | yahL → / → yahM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 344,381 | 0 | G | A | 98.2% | 172.1 / ‑4.1 | 55 | intergenic (+166/‑247) | yahL/yahM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (21/33); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAT > W3110S.gb/344314‑344448 | ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:1340764/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:1015195/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:372785/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:3556545/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:2863900/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:2726299/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:4128689/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:245307/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:2069882/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:1680195/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:1508896/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAg > 1:3396046/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAg > 1:1460799/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAg > 1:411515/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAg > 1:1578777/1‑71 (MQ=255) ccGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGgtg < 1:4056651/71‑1 (MQ=255) tgATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGAtc > 1:2040253/1‑71 (MQ=255) tgATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGAtc > 1:1391847/1‑71 (MQ=255) tCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCa > 1:3765034/1‑71 (MQ=255) tCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCa > 1:1826288/1‑71 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:1599113/71‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:2548806/71‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:2723588/71‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:1776503/71‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:3904021/71‑1 (MQ=255) gCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:2041108/70‑1 (MQ=255) acacTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCgg < 1:3187533/46‑1 (MQ=255) acacTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCgg < 1:555870/46‑1 (MQ=255) ttAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:3514556/65‑1 (MQ=255) ttAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:2426281/65‑1 (MQ=255) ttAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:2326061/65‑1 (MQ=255) ttAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:2091506/65‑1 (MQ=255) ttAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:820485/65‑1 (MQ=255) tAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATg < 1:1362110/57‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:931486/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:3735562/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:37816/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:700664/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:3433280/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:1073992/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:189942/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:2652837/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:2837184/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:3121184/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:3666771/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGATTtgtg < 1:6247/69‑1 (MQ=255) aCCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttgg < 1:3819198/71‑1 (MQ=255) aCCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttgg < 1:2703034/71‑1 (MQ=255) aCCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttgg < 1:2971083/71‑1 (MQ=255) aCCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttgg < 1:2525706/71‑1 (MQ=255) aCCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttgg < 1:1636121/71‑1 (MQ=255) ccAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:717434/60‑1 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAt > 1:4116040/1‑71 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAt > 1:4120717/1‑71 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAt > 1:1758780/1‑71 (MQ=255) | CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAT > W3110S.gb/344314‑344448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |