Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,636,526	C→T	L261L (CTG→CTA)	yfgL ←	protein assembly complex, lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,636,526	0	C	T	100.0%	272.1 / NA	86	L261L (CTG→CTA)	yfgL	protein assembly complex, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/40);  total (46/40)

TTCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGG  >  W3110S.gb/2636463‑2636583                                                                |                                                          ttCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAgcgc                                                      >  1:2825159/1‑69 (MQ=255) ttCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAgcgc                                                      >  1:656811/1‑69 (MQ=255)   cgcgTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGaa                                                   >  1:2496876/1‑70 (MQ=255)         tCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACg                                            >  1:1311691/1‑71 (MQ=255)                  tCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGaaaa                                                 <  1:1950365/57‑1 (MQ=255)                       ccACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAacgac                              <  1:696376/71‑1 (MQ=255)                       ccACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAacgac                              <  1:2829124/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTTGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:1540263/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:457582/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:2141572/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:2214043/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:2218776/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:1037414/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:2439490/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:3835923/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:292352/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:339261/71‑1 (MQ=255)                         aCTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg                            <  1:1217345/71‑1 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:1673522/1‑71 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:1782208/1‑71 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:3422898/1‑71 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:705660/1‑71 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:801564/1‑71 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:733582/1‑71 (MQ=255)                            gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAg                         >  1:3484938/1‑71 (MQ=255)                                 gATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac                                                <  1:750548/43‑1 (MQ=255)                                 gATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac                                                <  1:3860450/43‑1 (MQ=255)                                 gATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    >  1:1803391/1‑71 (MQ=255)                                 gATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    >  1:2011246/1‑71 (MQ=255)                                 gATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    >  1:1386597/1‑71 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACACCGACGGGAGTCgtg                    <  1:647784/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:777836/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:3428042/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:1263417/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:262613/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:4102138/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:3261648/70‑1 (MQ=255)                                  aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:1522376/70‑1 (MQ=255)                                  aTCAAGAGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:2114213/70‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:112976/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:3370903/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:3298644/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:2600658/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:2590777/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:2544210/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:2398650/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:2266618/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:1888855/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:1695182/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGt                                        >  1:16197/1‑49 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    <  1:3226407/69‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:40255/71‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:3445609/71‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:1420625/71‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:3101395/71‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:1729420/71‑1 (MQ=255)                                   tCAAGCGCCGTCAGGTTACAATTATAGGCTAGCGCGAAaacaa                                              >  1:1269228/1‑43 (MQ=255)                                   tCAAGCCCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:898636/71‑1 (MQ=255)                                    cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:2936123/70‑1 (MQ=255)                                    cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc                  <  1:3842586/70‑1 (MQ=255)                                             tCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa                <  1:3260163/63‑1 (MQ=255)                                             tCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa                <  1:871858/63‑1 (MQ=255)                                             tCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa                <  1:2264937/63‑1 (MQ=255)                                             tCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa                <  1:2934697/63‑1 (MQ=255)                                             tCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa                <  1:336806/63‑1 (MQ=255)                                             tCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa                <  1:3558334/63‑1 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGGCGGGAGTCGTGTCAACa              >  1:2339265/1‑63 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgt                     >  1:1405120/1‑56 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCa                 >  1:1137264/1‑60 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAAc               >  1:3783875/1‑62 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAAc               >  1:3399927/1‑62 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACa              >  1:3239960/1‑63 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACa              >  1:156081/1‑63 (MQ=255)                                               aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACa              >  1:1227031/1‑63 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGCCGGGAGTCGTGTCAACATCGCTCAGACg   >  1:2747733/1‑70 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGAGGGGAGTCGTGTCAAc               >  1:3867851/1‑58 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg                    >  1:1576408/1‑53 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAAc               >  1:2427796/1‑58 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCg           >  1:2636607/1‑62 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGGTCAGACg   >  1:1423624/1‑70 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACgg  >  1:1093456/1‑71 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACgg  >  1:746005/1‑71 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACgg  >  1:2012218/1‑71 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACgg  >  1:2437686/1‑71 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg   >  1:1184281/1‑70 (MQ=255)                                                   tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg   >  1:668820/1‑70 (MQ=255)                                                                |                                                          TTCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGG  >  W3110S.gb/2636463‑2636583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑