| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,688,036 | T→C | I43V (ATT→GTT) | yfhG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,688,036 | 0 | T | C | 96.0% | 253.7 / ‑0.5 | 75 | I43V (ATT→GTT) | yfhG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (24/48); total (24/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGA > W3110S.gb/2687970‑2688103 | tagcTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:757737/67‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:89735/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:72138/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:3726381/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:348565/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:307469/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:1025725/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:1503068/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:2571229/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtt < 1:2310334/70‑1 (MQ=255)tACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGGCAGCCAGTTGATAAACCGGAActtt < 1:1191587/70‑1 (MQ=255) cTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:1042375/71‑1 (MQ=255) ttGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttctt > 1:872561/1‑71 (MQ=255) ttGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttctt > 1:79289/1‑71 (MQ=255) ttGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttctt > 1:445551/1‑71 (MQ=255) ttGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct > 1:2664830/1‑70 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:1093041/70‑1 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:268663/70‑1 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:1482633/70‑1 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:521833/70‑1 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:2204841/70‑1 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:1982308/70‑1 (MQ=255) gCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:1982492/70‑1 (MQ=255) cAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:3042352/69‑1 (MQ=255) cAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttcttc < 1:541325/69‑1 (MQ=255) cccAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGc < 1:3794280/71‑1 (MQ=255) cccAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGc < 1:3789823/71‑1 (MQ=255) cccAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGc < 1:1284988/71‑1 (MQ=255) cccAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGc < 1:1976512/71‑1 (MQ=255) ccAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGc < 1:1154656/70‑1 (MQ=255) ccAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGAT‑AACCGGAACTTTTTCTTCTGCTGGCGt < 1:1562622/71‑1 (MQ=255) cAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAGCTTTTTCTTCTGCTGGCGt < 1:534628/71‑1 (MQ=255) cAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGt < 1:2343146/71‑1 (MQ=255) aGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTa < 1:3797562/71‑1 (MQ=255) aGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTa < 1:1449183/71‑1 (MQ=255) aGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTa < 1:185248/71‑1 (MQ=255) gCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAAtgg > 1:2205688/1‑71 (MQ=255) gCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAAtg > 1:559579/1‑70 (MQ=255) aGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAAtggctg < 1:3765106/65‑1 (MQ=255) aGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAAtggctg < 1:3720327/65‑1 (MQ=255) aGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTAt < 1:3916039/71‑1 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:2660585/41‑1 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:313214/41‑1 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:273821/41‑1 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:3582712/41‑1 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:2509114/41‑1 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAAtggctggc > 1:1225607/1‑65 (MQ=255) aGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttct < 1:2053040/40‑1 (MQ=255) aaaGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:1825230/71‑1 (MQ=255) aaaGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:3988709/71‑1 (MQ=255) aaaGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:1709923/71‑1 (MQ=255) aaaGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:1705177/71‑1 (MQ=255) aaaGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:683320/71‑1 (MQ=255) aaGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:2529095/70‑1 (MQ=255) aTAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCt > 1:3971812/1‑71 (MQ=255) aTAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCt > 1:3373293/1‑71 (MQ=255) aTAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCt > 1:2775243/1‑71 (MQ=255) aTAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCt > 1:473670/1‑71 (MQ=255) aTAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCt > 1:695237/1‑71 (MQ=255) aTAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCt > 1:3725595/1‑71 (MQ=255) cAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAAc > 1:2958737/1‑71 (MQ=255) cAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAAc < 1:761574/71‑1 (MQ=255) cAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAAc > 1:1892884/1‑71 (MQ=255) cAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAAc > 1:777480/1‑71 (MQ=255) cAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAAc > 1:2094258/1‑71 (MQ=255) gTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:3190844/56‑1 (MQ=255) gTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:1913011/56‑1 (MQ=255) gTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:2543820/56‑1 (MQ=255) gTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa < 1:4094778/56‑1 (MQ=255) tGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:103554/1‑71 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:2584236/1‑70 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:2951402/1‑70 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:1140629/1‑70 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:1609564/1‑70 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:1853146/1‑70 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:1389552/1‑70 (MQ=255) aTAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:1934799/70‑1 (MQ=255) aTAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:1478986/70‑1 (MQ=255) aTAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:3153660/70‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:3109278/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:509580/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:3711317/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:164128/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:2322398/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:1803584/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:165811/71‑1 (MQ=255) | TACCTTGCAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGA > W3110S.gb/2687970‑2688103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |