Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,697,845	G→A	G144D (GGT→GAT)	yfhH →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,697,845	0	G	A	100.0%	237.1 / NA	76	G144D (GGT→GAT)	yfhH	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/27);  total (49/27)

CTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGA  >  W3110S.gb/2697778‑2697911                                                                    |                                                                   cTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCt                                                                 <  1:1069457/71‑1 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGGTTCGGATCTGGTGGCGCAAAAc                                                    >  1:1803713/1‑71 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt                                                              >  1:920817/1‑61 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt                                                              >  1:3936584/1‑61 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt                                                              >  1:2952601/1‑61 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt                                                              >  1:2569301/1‑61 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCaaaa                                                     >  1:2343722/1‑70 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAAc                                                    >  1:2677636/1‑71 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAAc                                                    >  1:2904849/1‑71 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAAc                                                    >  1:1864757/1‑71 (MQ=255)              tAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAAc                                                    >  1:1329741/1‑71 (MQ=255)                        gcgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTgg                                           >  1:856226/1‑70 (MQ=255)                        gcgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTgg                                           >  1:2637171/1‑70 (MQ=255)                        gcgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTgg                                           >  1:3499017/1‑70 (MQ=255)                        gcgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTgg                                           >  1:1397161/1‑70 (MQ=255)                        gcgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTgg                                           >  1:2026422/1‑70 (MQ=255)                              tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTg                                                <  1:2840014/59‑1 (MQ=255)                              tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTg                                                <  1:2844844/59‑1 (MQ=255)                              tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTg                                                <  1:3903744/59‑1 (MQ=255)                                  cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa                                <  1:2036250/71‑1 (MQ=255)                                  cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa                                <  1:2301824/71‑1 (MQ=255)                                   ggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa                                <  1:1462825/70‑1 (MQ=255)                                   ggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa                                <  1:3202951/70‑1 (MQ=255)                                   ggcggATAATTCTGACCGGTATTGGCGCTACGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa                                <  1:3293016/70‑1 (MQ=255)                                      ggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAATTTTg                                                <  1:571965/51‑1 (MQ=255)                                       gATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTg                           <  1:418024/71‑1 (MQ=255)                                       gATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTg                           <  1:4028454/71‑1 (MQ=255)                                       gATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTg                           <  1:1531452/71‑1 (MQ=255)                                       gATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTg                           <  1:520715/71‑1 (MQ=255)                                       gATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTg                           <  1:1455162/71‑1 (MQ=255)                                       gATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTg                           <  1:16210/71‑1 (MQ=255)                                                       aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc                      >  1:3536519/1‑60 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAg                               <  1:111993/50‑1 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:653354/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:1490111/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:1297359/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:3136777/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:3642906/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:1133910/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:45632/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2719075/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:1544314/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:160628/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:1372300/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2416809/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2426530/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2555116/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:136522/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2595744/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2612484/1‑69 (MQ=255)                                                        ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg            >  1:2744388/1‑69 (MQ=255)                                                          ggCGCTTCGGATCTGGTTGCGCAAAACTTTGCCTGGAAGCtg                                     <  1:3335123/42‑1 (MQ=38)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtg                                     <  1:3011003/42‑1 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtg                                     <  1:2442065/42‑1 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtg                                     <  1:684596/42‑1 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGAt                             >  1:3078836/1‑50 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGAt                             >  1:3694308/1‑50 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGAt                             >  1:3706957/1‑50 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGAt                             >  1:4065310/1‑50 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGAt                             >  1:2351936/1‑50 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:129956/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:3183247/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:2652056/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:850544/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:739697/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:2158433/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:3253222/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:2631156/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:3305679/1‑71 (MQ=255)                                                          ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt        >  1:2579005/1‑71 (MQ=255)                                                              cTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTgcgc    <  1:2899975/71‑1 (MQ=255)                                                              cTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTgcgc    <  1:1841630/71‑1 (MQ=255)                                                              cTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTgcgc    <  1:2511885/71‑1 (MQ=255)                                                                 cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa  <  1:2102350/70‑1 (MQ=255)                                                                 cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa  <  1:604720/70‑1 (MQ=255)                                                                 cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa  <  1:394575/70‑1 (MQ=255)                                                                    |                                                                   CTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGA  >  W3110S.gb/2697778‑2697911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑