Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,717,229	G→A	intergenic (‑45/‑162)	yfiF ← / → trxC	predicted methyltransferase/thioredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,717,229	0	G	A	100.0%	67.6 / NA	25	intergenic (‑45/‑162)	yfiF/trxC	predicted methyltransferase/thioredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/6);  total (19/6)

CCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAA  >  W3110S.gb/2717165‑2717290                                                                 |                                                              ccTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCcgcg                                                         >  1:4069344/1‑71 (MQ=255)    ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCgg                                                      >  1:1196109/1‑71 (MQ=255)    ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCgg                                                      >  1:604639/1‑71 (MQ=255)    ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCgg                                                      >  1:2714986/1‑71 (MQ=255)    ttcatttcatCGTTCACGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCgg                                                      >  1:3622447/1‑71 (MQ=255)            atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                              <  1:2512717/71‑1 (MQ=255)            atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                              <  1:2764192/71‑1 (MQ=255)            atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                              <  1:3850364/71‑1 (MQ=255)            atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                              <  1:397104/71‑1 (MQ=255)                 tCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCa                                               >  1:627132/1‑65 (MQ=255)                 tCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCa                                               >  1:3918157/1‑65 (MQ=255)                                  aGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCGATGAttt                                         >  1:512406/1‑54 (MQ=255)                                    cgcTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                              <  1:3120426/47‑1 (MQ=255)                                     gcTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGAtt                                          >  1:1214061/1‑50 (MQ=255)                                     gcTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTa                     >  1:1792029/1‑71 (MQ=255)                                     gcTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTa                     >  1:2922057/1‑71 (MQ=255)                                      cTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt                    <  1:1960646/71‑1 (MQ=255)                                           cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAg                               >  1:452359/1‑55 (MQ=255)                                           cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAc               >  1:1121509/1‑71 (MQ=255)                                           cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAc               >  1:853860/1‑71 (MQ=255)                                           cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAc               >  1:3939192/1‑71 (MQ=255)                                           cgAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAc               >  1:3262628/1‑71 (MQ=255)                                                       aTTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa    >  1:1658899/1‑70 (MQ=255)                                                       aTTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACC‑AAGCCTGCGACTATCATACCTATTGAATaaaa  >  1:1675405/1‑71 (MQ=255)                                                        ttACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa  >  1:3332489/1‑71 (MQ=255)                                                                 |                                                              CCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAA  >  W3110S.gb/2717165‑2717290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑