| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,732,036 | T→A | D265V (GAT→GTT) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,732,036 | 0 | T | A | 96.5% | 80.3 / ‑3.6 | 29 | D265V (GAT→GTT) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (18/10); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
CCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAGCGCGCC > W3110S.gb/2731970‑2732103 | cccGCGCCGACCTTGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGtt < 1:1127243/71‑1 (MQ=255)cccGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGtt < 1:3516331/71‑1 (MQ=255)cccGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGtt < 1:3382164/71‑1 (MQ=255) ccGCGCCGACCATGGTATGTATTTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGtt < 1:877241/70‑1 (MQ=255) gTATGTAATTCGTCGATAAATAGGATGGCGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTa < 1:2821292/70‑1 (MQ=255) gTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTa < 1:2378000/70‑1 (MQ=255) gTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTa < 1:3499730/70‑1 (MQ=255) tATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTa < 1:187214/69‑1 (MQ=255) tGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACg > 1:2979056/1‑71 (MQ=255) tAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGtt > 1:1754595/1‑71 (MQ=255) tAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGtt > 1:3512218/1‑71 (MQ=255) tAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGt > 1:265272/1‑70 (MQ=255) tAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGt > 1:2663197/1‑70 (MQ=255) aaTTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttc > 1:1542211/1‑71 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:1974663/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:3569292/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:143956/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:855506/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:3210722/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:3177976/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:2964418/1‑70 (MQ=255) cgtcgATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGttcttc > 1:2313218/1‑70 (MQ=255) gCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGccc > 1:1345392/1‑71 (MQ=255) gCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGccc > 1:665896/1‑71 (MQ=255) gCCTTCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGccc > 1:3441262/1‑71 (MQ=255) ttCCTGTTTGGCAAGAACGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTAGCCCCAg > 1:417462/1‑71 (MQ=255) cAAGAACGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAgcgcg < 1:810634/71‑1 (MQ=255) cAAGAACGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAgcgcg < 1:2009873/71‑1 (MQ=255) aGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAGCGCGcc < 1:958790/71‑1 (MQ=255) | CCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACGTTGCCTTCCTGTTTGGCAAGATCGTTAAGCACGCCTTTTAAACGTTCTTCAAACTCACCGCGATATTTCGCCCCAGCCACCAGCGCGCC > W3110S.gb/2731970‑2732103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |