Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,773,435	T→C	L488L (TTG→CTG)	yfjW →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,773,435	0	T	C	100.0%	161.0 / NA	45	L488L (TTG→CTG)	yfjW	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

AGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTG  >  W3110S.gb/2773367‑2773492                                                                     |                                                          aGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg                                                         <  1:885148/71‑1 (MQ=255)      aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg                                                     <  1:2794013/70‑1 (MQ=255)          tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc                                                  >  1:1048668/1‑69 (MQ=255)          tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc                                                  >  1:1415378/1‑69 (MQ=255)          tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc                                                  >  1:3577441/1‑69 (MQ=255)          tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc                                                  >  1:3967289/1‑69 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAg                                     >  1:2465081/1‑61 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:2453513/1‑70 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:4111438/1‑70 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:486742/1‑70 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:2467333/1‑70 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:2449724/1‑70 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:2446618/1‑70 (MQ=255)                               tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAAt                            >  1:678353/1‑70 (MQ=255)                                   gCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:812321/71‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:2204788/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:2049696/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:1875164/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:2582627/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:810480/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:270495/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:2871769/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:3485426/67‑1 (MQ=255)                                       gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       <  1:3250933/67‑1 (MQ=255)                                            ggATATTATTATTTCTGAAGGGAGTCTGTATGGACTa                                                <  1:536154/37‑1 (MQ=38)                                            ggAGATTATTATTTCTGAAGGGAGTCTGTATGGACTa                                                <  1:2513048/37‑1 (MQ=255)                                             gagaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa                         <  1:1353961/59‑1 (MQ=255)                                              agaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGt                                              >  1:1052481/1‑37 (MQ=255)                                              agaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGt                                              >  1:1269634/1‑37 (MQ=255)                                                  tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATc                           >  1:1835432/1‑52 (MQ=255)                                                  tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat                    >  1:3343499/1‑59 (MQ=255)                                                  tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat                    >  1:1555550/1‑59 (MQ=255)                                                  tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat                    >  1:394905/1‑59 (MQ=255)                                                  tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat                    >  1:2696716/1‑59 (MQ=255)                                                  tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat                    >  1:3194470/1‑59 (MQ=255)                                                    ttattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATgg                <  1:652230/61‑1 (MQ=255)                                                    ttattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATgg                <  1:1606544/61‑1 (MQ=255)                                                     tattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGAt      <  1:2348152/70‑1 (MQ=255)                                                     tattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGAt      <  1:3491661/70‑1 (MQ=255)                                                     tattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGAt      <  1:2965395/70‑1 (MQ=255)                                                     tattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATc     >  1:2668388/1‑71 (MQ=255)                                                      attTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGca              >  1:2581693/1‑61 (MQ=255)                                                        ttCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       >  1:2558069/1‑50 (MQ=255)                                                        ttCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaaaa                       >  1:883041/1‑50 (MQ=255)                                                         tCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTg  <  1:783055/70‑1 (MQ=255)                                                                     |                                                          AGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTG  >  W3110S.gb/2773367‑2773492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑