| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,794,102 | G→A | L398L (TTG→TTA) | gabP → | gamma‑aminobutyrate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,794,102 | 0 | G | A | 84.4% | 227.8 / 33.2 | 90 | L398L (TTG→TTA) | gabP | gamma‑aminobutyrate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/14); new base A (39/37); total (39/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGT > W3110S.gb/2794035‑2794167 | taGTCATCGCCGTTTCACAGTTGCGGATGCGCGAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:936107/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:505939/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:2252615/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:2449934/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:2501246/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:2554488/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:1819149/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:3297950/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:391895/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:570028/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:1497102/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:733160/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:82580/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:827057/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:1182614/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:1141005/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:111051/1‑70 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCCGCGAGCAGAAGGAAGCGAAATTCGCTTACg > 1:563833/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:1103146/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:770474/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:3223878/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:2533004/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:2422509/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:231393/1‑70 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt < 1:2241975/70‑1 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtc > 1:1805361/1‑69 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCAAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt > 1:2035920/1‑70 (MQ=255) cacaGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATcc < 1:2672905/70‑1 (MQ=255) cacaGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATcc < 1:3284242/70‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:1966560/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:2711196/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:2937997/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:1841119/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:3042135/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:3370357/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:3464977/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:1502060/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:149902/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:599413/71‑1 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:82538/71‑1 (MQ=255) gTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:2990813/70‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGcc < 1:1014531/50‑2 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCg < 1:2409065/50‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCg < 1:2859107/50‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCg < 1:2878719/50‑1 (MQ=255) tGCGGATGCGCAAAATTCTGCTAGCAGAAGGAAGCGAAATTCGCTTGCg < 1:3386198/49‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa < 1:2181630/70‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa < 1:4087608/70‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa < 1:1830844/70‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa < 1:2651810/70‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt < 1:1716102/52‑1 (MQ=255) cgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa < 1:3668835/64‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:2117724/71‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:2086363/71‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:524018/71‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:1401370/71‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:4041808/71‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:2025283/71‑1 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:92787/71‑1 (MQ=255) cAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:1823012/70‑1 (MQ=255) aaaaTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:209946/69‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:2519412/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:588953/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:574334/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:548775/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:817303/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:426498/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:3235925/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:3907660/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:3678449/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:1954770/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:3365780/50‑1 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:3428670/50‑1 (MQ=255) tCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTa > 1:3149354/1‑40 (MQ=255) tCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTa > 1:3408948/1‑40 (MQ=255) tCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTa > 1:2027160/1‑40 (MQ=255) tCGCTTAAGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc < 1:2500861/49‑1 (MQ=39) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:1253198/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:2787262/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:2817266/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:269644/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:602969/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:1891828/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:169/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:3911963/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:3793091/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt > 1:3629284/1‑50 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAg > 1:543962/1‑70 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGt > 1:1299639/1‑71 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGt > 1:1211683/1‑71 (MQ=255) | TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGT > W3110S.gb/2794035‑2794167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |