Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,821,480	T→C	T316A (ACC→GCC)	recA ←	DNA strand exchange and recombination protein with protease and nuclease activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,821,480	0	T	C	100.0%	225.4 / NA	65	T316A (ACC→GCC)	recA	DNA strand exchange and recombination protein with protease and nuclease activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/58);  total (7/58)

GCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACC  >  W3110S.gb/2821420‑2821540                                                             |                                                             gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:1144377/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:2368048/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:24702/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3381942/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3615933/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:2011638/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:1990017/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3944013/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3232126/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3110924/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:1396346/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3174194/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:1167358/71‑1 (MQ=255) gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCCGCTTCCGGGTTa                                                    <  1:1969154/71‑1 (MQ=255)    ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt                                                 <  1:1593271/71‑1 (MQ=255)    ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt                                                 <  1:1680099/71‑1 (MQ=255)    ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt                                                 <  1:3081508/71‑1 (MQ=255)    ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt                                                 <  1:1782713/71‑1 (MQ=255)    ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt                                                 <  1:3008991/71‑1 (MQ=255)    ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt                                                 <  1:3125782/71‑1 (MQ=255)      gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCttt                                               >  1:1568149/1‑71 (MQ=255)      gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCtt                                                >  1:1661466/1‑70 (MQ=255)      gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCtt                                                >  1:2914363/1‑70 (MQ=255)                 gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3436204/55‑1 (MQ=255)                 gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:3167078/55‑1 (MQ=255)                 gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa                                                    <  1:586795/55‑1 (MQ=255)                  cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt                                   <  1:731052/71‑1 (MQ=255)                  cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt                                   <  1:1212714/71‑1 (MQ=255)                  cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt                                   <  1:1461468/71‑1 (MQ=255)                  cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt                                   <  1:2964651/71‑1 (MQ=255)                  cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt                                   <  1:1808679/71‑1 (MQ=255)                       cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt                                                      <  1:2374377/47‑1 (MQ=255)                       cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt                                                      <  1:396927/47‑1 (MQ=255)                       cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt                                                      <  1:243205/47‑1 (MQ=255)                           aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          <  1:358888/71‑1 (MQ=255)                           aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          <  1:2682280/71‑1 (MQ=255)                           aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          <  1:2135486/71‑1 (MQ=255)                           aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          <  1:3568754/71‑1 (MQ=255)                           aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          <  1:1600724/71‑1 (MQ=255)                           aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          >  1:3463870/1‑71 (MQ=255)                           aaCTCACGTACTTTATTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc                          <  1:2374983/71‑1 (MQ=255)                            aCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt                                                      <  1:87994/42‑1 (MQ=255)                                aCGTAGTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                      <  1:2278874/70‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                      <  1:1081346/70‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                      <  1:3825101/70‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:2106427/71‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:73230/71‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:1211863/71‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:183287/71‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:2132732/71‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:2289364/71‑1 (MQ=255)                                aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc                     <  1:3282354/71‑1 (MQ=255)                                 cGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc                    <  1:3664140/71‑1 (MQ=255)                                 cGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc                    <  1:3245673/71‑1 (MQ=255)                                               gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct         <  1:2595485/68‑1 (MQ=255)                                               gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct         <  1:3931460/68‑1 (MQ=255)                                               gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct         <  1:54108/68‑1 (MQ=255)                                                aTCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                      <  1:762002/54‑1 (MQ=255)                                                  ctctTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAccc                   >  1:1784617/1‑55 (MQ=255)                                                  ctctTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc              >  1:3107310/1‑60 (MQ=255)                                                  ctctTTCGCGGCTTCCGGGTTATCTTTCAGCAAGGCAGTCGCATTCGCTTTACCCTGAcc              >  1:1680828/1‑60 (MQ=255)                                                   tctTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt                                   <  1:3111905/38‑1 (MQ=255)                                                     tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAcc  <  1:3477776/69‑1 (MQ=255)                                                     tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAcc  <  1:3429902/69‑1 (MQ=255)                                                     tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAcc  <  1:2816280/69‑1 (MQ=255)                                                             |                                                             GCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACC  >  W3110S.gb/2821420‑2821540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑