Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,840,761	A→G	E372E (GAA→GAG)	ascB →	cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,840,761	0	A	G	100.0%	146.0 / NA	41	E372E (GAA→GAG)	ascB	cryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/34);  total (7/34)

GGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGC  >  W3110S.gb/2840699‑2840827                                                               |                                                                   ggTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGc                                                              <  1:2005598/69‑1 (MQ=255)                          tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:3260219/71‑1 (MQ=255)                            tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa                                                                  >  1:1065888/1‑38 (MQ=255)                            tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa                                                                  >  1:2850434/1‑38 (MQ=255)                            tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa                                                                  >  1:3097283/1‑38 (MQ=255)                            tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa                                                                  >  1:1407212/1‑38 (MQ=255)                            tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa                                                                  >  1:4017348/1‑38 (MQ=255)                              cGATCGTTATCAGAAGCCGCTGTTTTTGGGGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATg                               <  1:2144341/71‑1 (MQ=255)                              cGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATg                               <  1:13813/71‑1 (MQ=255)                              cGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATg                               <  1:3082701/71‑1 (MQ=255)                              cGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGATGCCAATg                               <  1:1855125/71‑1 (MQ=255)                                   gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGa                                             <  1:2073074/52‑1 (MQ=255)                                   gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga                           <  1:3537251/70‑1 (MQ=255)                                   gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga                           <  1:1600676/70‑1 (MQ=255)                                   gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga                           <  1:2540800/70‑1 (MQ=255)                                   gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga                           <  1:226969/70‑1 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGTACGGCCTGGGCGCAAAAGATGAAt                                           >  1:1417980/1‑52 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt                                           >  1:4001681/1‑52 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt                                           >  1:3595421/1‑52 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt                                           >  1:2857927/1‑52 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt                                           >  1:800316/1‑52 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt                                           >  1:2106840/1‑52 (MQ=255)                                     tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt                                           >  1:1654613/1‑52 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATTGCgag                          <  1:3406918/67‑1 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:2211708/67‑1 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:615767/67‑1 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:2517215/67‑1 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:2138322/67‑1 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:1973337/67‑1 (MQ=255)                                       tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:3553899/67‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTTGGCGCAAAAGATGAATTTGCTGcc                                   <  1:3452970/54‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:1011424/54‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:1473390/54‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:3554924/54‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:3250681/54‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:2787437/54‑1 (MQ=255)                                           aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc                                   <  1:2151167/54‑1 (MQ=255)                                             gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgtcg                <  1:1469173/71‑1 (MQ=255)                                             gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgacg                <  1:1703538/71‑1 (MQ=255)                                             gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgacg                <  1:1473955/71‑1 (MQ=255)                                             gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgacg                <  1:1077832/71‑1 (MQ=255)                                                gCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATgta                                            <  1:2328201/40‑3 (MQ=39)                                                        tggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag                          <  1:496681/50‑1 (MQ=255)                                                           tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCTGc  <  1:719298/71‑1 (MQ=255)                                                           tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGc  <  1:1719696/71‑1 (MQ=255)                                                           tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGc  <  1:2921071/71‑1 (MQ=255)                                                               |                                                                   GGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGC  >  W3110S.gb/2840699‑2840827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑