| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,860,110 | G→A | V9I (GTC→ATC) | ygbJ → | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,860,110 | 0 | G | A | 76.4% | 102.6 / 32.2 | 55 | V9I (GTC→ATC) | ygbJ | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (5/8); new base A (3/39); total (8/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGT > W3110S.gb/2860043‑2860172 | acaATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTc < 1:958810/70‑1 (MQ=255)acaATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATc < 1:984958/70‑1 (MQ=255)acaATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATc < 1:2768981/70‑1 (MQ=255)acaATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATc < 1:367891/70‑1 (MQ=255) atatCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTa < 1:2424547/71‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATc < 1:98378/70‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATc < 1:1756478/70‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATc < 1:373583/70‑1 (MQ=255) atacaaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATc < 1:3961127/66‑1 (MQ=255) aaacaaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGtt < 1:1080857/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:1330526/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:337701/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:2161782/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:2069597/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:2783176/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:4119261/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:767486/71‑1 (MQ=255) aaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGAGCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:1867761/71‑1 (MQ=255) atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:179944/71‑1 (MQ=255) atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:977983/71‑1 (MQ=255) atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:1773244/71‑1 (MQ=255) atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:872419/71‑1 (MQ=255) atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:901232/71‑1 (MQ=255) tatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:3865540/70‑1 (MQ=255) tatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAgg < 1:1869845/71‑1 (MQ=255) aCTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc < 1:242936/70‑1 (MQ=255) aCTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc < 1:3166286/70‑1 (MQ=255) cTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc < 1:2835235/69‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:4108481/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:2612730/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:1231815/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:1450730/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:1653206/71‑1 (MQ=255) aTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCgatggg < 1:1997070/70‑1 (MQ=255) cAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgggaatggg < 1:1135599/71‑1 (MQ=255) aGGAGAGCAGATGAATACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAg < 1:2304226/53‑1 (MQ=255) aGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgggaatggg < 1:2706725/69‑1 (MQ=255) gCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatgggaa > 1:2976509/1‑59 (MQ=255) gCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatgggaa > 1:2256513/1‑59 (MQ=255) gCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatgggaa > 1:2086265/1‑59 (MQ=255) gCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatgggaa > 1:2574684/1‑59 (MQ=255) gCAGATGAAAACGGGATCTGAGATTCATGTCGGTATCGTTgg > 1:3293763/1‑42 (MQ=255) aGATGAAAACGGGATCTGAGTTTCATATCGGTATCGtt < 1:739902/38‑1 (MQ=255) gggATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:2599528/45‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAgg < 1:3807864/70‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAgg < 1:1736618/70‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAgg < 1:3346834/70‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAgg < 1:2942029/70‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAgg < 1:1436061/70‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGt < 1:1918762/71‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGt < 1:3961377/71‑1 (MQ=255) aGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGt < 1:1764156/71‑1 (MQ=255) cATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagcagc > 1:3191608/1‑44 (MQ=255) cATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCAc > 1:186213/1‑46 (MQ=255) cATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCAc > 1:1529374/1‑46 (MQ=255) | ACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGT > W3110S.gb/2860043‑2860172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |