| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,867,123 | G→A | P96L (CCG→CTG) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,867,123 | 0 | G | A | 91.4% | 101.4 / 1.7 | 35 | P96L (CCG→CTG) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (6/26); total (8/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
TAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTG > W3110S.gb/2867057‑2867190 | taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:915918/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:4060250/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:4038176/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:4033476/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:322130/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:2313128/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:2727595/71‑1 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg < 1:2715626/71‑1 (MQ=255) cgtttCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:661692/69‑1 (MQ=255) ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGa < 1:3788227/69‑1 (MQ=255) ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:3185696/71‑1 (MQ=255) ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:2672252/71‑1 (MQ=255) ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:241030/71‑1 (MQ=255) cTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:2673474/70‑1 (MQ=255) cTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:740218/70‑1 (MQ=255) tACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTg < 1:3775995/62‑1 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgt > 1:3835593/1‑71 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgt > 1:1145407/1‑71 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgt > 1:496404/1‑71 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgt > 1:1527367/1‑71 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgt > 1:2843303/1‑71 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgt > 1:177481/1‑71 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:2489027/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:3376621/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:3319031/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:139248/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:465180/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:2129999/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:1322674/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:243603/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGAtt < 1:1009583/63‑1 (MQ=255) taGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCAGAGCTACTGGCTGCACTGGCTGGAtt < 1:325065/63‑1 (MQ=255) cATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGCtt < 1:1766878/43‑1 (MQ=38) ccGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGctgctg > 1:3662644/1‑70 (MQ=255) ccGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGctgctg > 1:662248/1‑70 (MQ=255) | TAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTG > W3110S.gb/2867057‑2867190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |