Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,910,539	C→A	R590L (CGC→CTC)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,910,539	0	C	A	100.0%	79.4 / NA	25	R590L (CGC→CTC)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/22);  total (3/22)

AGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGC  >  W3110S.gb/2910471‑2910595                                                                     |                                                         agcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAgg                                                        <  1:734652/71‑1 (MQ=255) agcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAgg                                                        <  1:3447665/71‑1 (MQ=255) agcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAgg                                                        <  1:2241646/71‑1 (MQ=255)  gcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGt                                                       <  1:3838162/71‑1 (MQ=255)   cagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGt                                                       <  1:747917/70‑1 (MQ=255)          cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggc                                               <  1:175077/71‑1 (MQ=255)          cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggc                                               <  1:3321728/71‑1 (MQ=255)          cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggc                                               <  1:2783797/71‑1 (MQ=255)                 gtgCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTa                                          >  1:335928/1‑69 (MQ=255)                 gtgCATCAGGTTGCCAACACATTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGc                                        >  1:2142549/1‑71 (MQ=255)                                 acacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTTCGGCGTGTAGCTTTTTTGCTGAAGt                          <  1:43733/69‑1 (MQ=255)                                 acacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGACGt                          <  1:1766391/69‑1 (MQ=255)                                 acacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGt                          <  1:1444257/69‑1 (MQ=255)                                  cacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcgga                                               <  1:403761/47‑2 (MQ=255)                                    ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg                        <  1:3073187/68‑1 (MQ=255)                                    ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg                        <  1:621669/68‑1 (MQ=255)                                    ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg                        <  1:2197758/68‑1 (MQ=255)                                    ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg                        <  1:1939513/68‑1 (MQ=255)                                                     aCCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTT‑CTTCAGCGCGGCGGCGTCCTg   <  1:4066058/71‑1 (MQ=255)                                                      ccGTTATCTTTACTTAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg   <  1:1964488/71‑1 (MQ=255)                                                      ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg   <  1:410496/71‑1 (MQ=255)                                                      ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg   <  1:199681/71‑1 (MQ=255)                                                      ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg   <  1:2628262/71‑1 (MQ=255)                                                      ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg   <  1:2462666/71‑1 (MQ=255)                                                                tACTGAGGTTTTGCGGCGTGTAGCTTTTTTTCTGAAGTTGCTTCAGCGCGGCGGCGTCCAGc  >  1:312100/1‑62 (MQ=255)                                                                     |                                                         AGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGC  >  W3110S.gb/2910471‑2910595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑