| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 393,168 | T→C | G310G (GGT→GGC) | yaiU → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 393,168 | 0 | T | C | 78.4% | 75.4 / 16.7 | 37 | G310G (GGT→GGC) | yaiU | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base C (6/23); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.78e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
ACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCA > W3110S.gb/393098‑393235 | acGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:3141066/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:61239/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:2138741/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:469953/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:1488144/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:3603521/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:1551797/71‑1 (MQ=255) ttgATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGtt < 1:161899/71‑1 (MQ=255) gTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGt < 1:2307610/71‑1 (MQ=255) gTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGt < 1:209313/71‑1 (MQ=255) gTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGt < 1:2035681/71‑1 (MQ=255) gTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGt < 1:2706812/71‑1 (MQ=255) gTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACCGCACTTACGTTGACGGt < 1:2683427/71‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGTAACGGt < 1:532732/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:2791680/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:2969945/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:2635165/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:3864522/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:2561875/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:2487240/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:452846/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:2512810/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:1046856/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:1950597/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:863147/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:858456/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:882889/53‑1 (MQ=255) gCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:1476850/53‑1 (MQ=255) ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTAc > 1:2036464/1‑53 (MQ=255) ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTAc > 1:2026399/1‑53 (MQ=255) ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTAc > 1:59200/1‑53 (MQ=255) ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTAc > 1:512695/1‑53 (MQ=255) ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTAc > 1:136965/1‑53 (MQ=255) ttGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTAc > 1:1546293/1‑53 (MQ=255) gAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGt < 1:3164499/49‑1 (MQ=255) tCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTgg > 1:769049/1‑71 (MQ=255) tCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTgg > 1:1474252/1‑71 (MQ=255) tCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTgg > 1:4113118/1‑71 (MQ=255) tCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTgg > 1:4112595/1‑71 (MQ=255) tCAACAACGACCTGTCTGCAACCATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTgg > 1:2696267/1‑71 (MQ=255) tCTGCAACCATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGa > 1:3093437/1‑70 (MQ=255) tGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAg > 1:1667125/1‑71 (MQ=255) tGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAg > 1:186422/1‑71 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:669987/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:904479/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:1202035/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:3277121/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:2045174/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:1860805/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGt < 1:1290147/71‑1 (MQ=255) ccATGAGCAACGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGGTTGAAAGCCGGt < 1:914820/71‑1 (MQ=255) cGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCa < 1:622457/71‑1 (MQ=255) cGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCa < 1:3713175/71‑1 (MQ=255) cGGCACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCa < 1:1491930/71‑1 (MQ=255) | ACGTCTTTGTTGATGGTAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACGGTACTTACGTTGACGGTAGCACCAACTCCGACGCTTGGGGCTTCGGTTTGAAAGCCGGTTACGACTTCA > W3110S.gb/393098‑393235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |