BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,013,681	G→A	V11V (GTC→GTT)	yqeC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,013,681	0	G	A	100.0%	546.4 / NA	169	V11V (GTC→GTT)	yqeC	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (84/85); total (84/85)

Only 100 of 169 total aligned reads displayed.

AGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTAT  >  W3110S.gb/3013614‑3013744

agcTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAAc                                                               >  1:1677693/1‑70 (MQ=255)

agcTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAAc                                                               >  1:2260715/1‑70 (MQ=255)

agcTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt                                                              <  1:3401814/71‑1 (MQ=255)

      ttttCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGcc                                                        <  1:3715674/71‑1 (MQ=255)

      ttttCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGcc                                                        <  1:2566072/71‑1 (MQ=255)

               cAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCa                                               <  1:807784/71‑1 (MQ=255)

                      aacaacAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACtat                                          >  1:587617/1‑69 (MQ=255)

                      aacaacAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATAc                                        >  1:2850712/1‑71 (MQ=255)

                         aacaGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACttt                                     >  1:2096975/1‑71 (MQ=255)

                             gAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCAc                                 >  1:1830920/1‑71 (MQ=255)

                                  cacaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            >  1:642612/1‑71 (MQ=255)

                                  cacaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            >  1:3161849/1‑71 (MQ=255)

                                  cacaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            >  1:3213156/1‑71 (MQ=255)

                                  cacaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            >  1:2774709/1‑71 (MQ=255)

                                  cacaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            >  1:1942000/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:718301/70‑1 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:886073/70‑1 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:3901462/70‑1 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:1915063/70‑1 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:2719643/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:2756971/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:3628958/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:2989226/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:1093639/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:872270/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCt                           >  1:1555403/1‑71 (MQ=255)

                                   acaGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGCTCAACTATACTTTTCACGAGTc                            >  1:2773309/1‑70 (MQ=255)

                                    caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGcc                                                        <  1:1036663/41‑1 (MQ=255)

                                    caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt                          <  1:3158152/71‑1 (MQ=255)

                                    caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt                          <  1:2201983/71‑1 (MQ=255)

                                    caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt                          <  1:438067/71‑1 (MQ=255)

                                    caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt                          <  1:4072875/71‑1 (MQ=255)

                                     aGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACtata                                         >  1:3915036/1‑55 (MQ=255)

                                     aGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACtata                                         >  1:2696127/1‑55 (MQ=255)

                                     aGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt                          >  1:2562438/1‑70 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:386177/66‑1 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:2900765/66‑1 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:4057506/66‑1 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:246874/66‑1 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:3565936/66‑1 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:1756012/66‑1 (MQ=255)

                                       tGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc                            <  1:3722056/66‑1 (MQ=255)

                                                    cgcACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTgg            <  1:2441938/69‑1 (MQ=255)

                                                    cgcACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTgg            <  1:2672596/69‑1 (MQ=255)

                                                    cgcACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTgg            <  1:3963678/69‑1 (MQ=255)

                                                    cgcACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTgg            <  1:1925158/69‑1 (MQ=255)

                                                    cgcACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGa          >  1:3483844/1‑71 (MQ=255)

                                                      cACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTgg            >  1:1328201/1‑67 (MQ=255)

                                                      cACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa        <  1:1662170/71‑1 (MQ=255)

                                                      cACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa        <  1:3672074/71‑1 (MQ=255)

                                                      cACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa        <  1:296702/71‑1 (MQ=255)

                                                       aCCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa        <  1:106968/70‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     <  1:3112125/55‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     <  1:2499617/55‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     <  1:3409173/55‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATaa       <  1:1510311/69‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     <  1:2036244/71‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     <  1:3263358/71‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     <  1:668150/71‑1 (MQ=255)

                                                         cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     <  1:2346999/71‑1 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTAt                       >  1:379529/1‑52 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTAt                       >  1:3963390/1‑52 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTAt                       >  1:2238097/1‑52 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATaaa      >  1:3456738/1‑69 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:3903374/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:1901450/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:2341077/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:2493408/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:1565564/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:68200/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:3089634/1‑70 (MQ=255)

                                                          tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:4098545/1‑70 (MQ=255)

                                                           aaaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     <  1:2807489/53‑1 (MQ=255)

                                                           aaaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTggg           >  1:1602688/1‑63 (MQ=255)

                                                           aaaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGa          >  1:3617345/1‑64 (MQ=255)

                                                           aaaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGa          >  1:1698086/1‑64 (MQ=255)

                                                           aaaTCAATAACTAATGCCGATGGGACAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGa          >  1:3329209/1‑64 (MQ=255)

                                                            aaTCAATAACTAATGCCGATTGGTCAACTATACTTTTCACGAGTCTTTATGAc                    <  1:235757/53‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTc                                   >  1:1259470/1‑38 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                    <  1:929926/53‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                    <  1:1299455/53‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAtt    >  1:449108/1‑69 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt     >  1:1178611/1‑68 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa   <  1:142691/70‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa   <  1:2178038/70‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa   <  1:3538668/70‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa   <  1:1141332/70‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa   <  1:2709935/70‑1 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa   >  1:3067414/1‑70 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTAt  >  1:1862771/1‑71 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTAt  >  1:1450794/1‑71 (MQ=255)

                                                            aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTAt  >  1:76533/1‑71 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATg                      >  1:1308573/1‑49 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     >  1:946003/1‑50 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     >  1:3035956/1‑50 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                     >  1:41405/1‑50 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAcc                   >  1:774219/1‑52 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACct                  >  1:3276983/1‑53 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACct                  >  1:3165745/1‑53 (MQ=255)

                                                              tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACct                  >  1:3039286/1‑53 (MQ=255)

AGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTAT  >  W3110S.gb/3013614‑3013744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`