BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,100,581	C→T	C70Y (TGC→TAC)	yggL ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,100,581	0	C	T	100.0%	222.8 / NA	69	C70Y (TGC→TAC)	yggL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (44/25); total (44/25)

GCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGATAACCTC  >  W3110S.gb/3100514‑3100648

gcgcTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAg                                                                   <  1:927248/70‑1 (MQ=255)

gcgcTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAg                                                                   <  1:1367130/70‑1 (MQ=255)

gcgcTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAg                                                                   <  1:313673/70‑1 (MQ=255)

gcgcTCTTCCAGCCACTTACGCACAATCGCCTGATGTACTTCGGTGCATTTGCCGATTTCATGCATGTAg                                                                   <  1:2959303/70‑1 (MQ=255)

      ttCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAg                                                              <  1:1921454/69‑1 (MQ=255)

      ttCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAg                                                              <  1:3798052/69‑1 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGAt                                                                 >  1:238070/1‑51 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:1077934/1‑52 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:2042936/1‑52 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:3229047/1‑52 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:1864284/1‑52 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:4067129/1‑52 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:839511/1‑52 (MQ=255)

                     cacaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATc                                                                >  1:925605/1‑52 (MQ=255)

                      acaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATa                                            <  1:3401174/71‑1 (MQ=255)

                      acaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATa                                            <  1:554098/71‑1 (MQ=255)

                      acaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATa                                            <  1:2254423/71‑1 (MQ=255)

                      acaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATa                                            <  1:2906376/71‑1 (MQ=255)

                      acaATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATa                                            <  1:2454040/71‑1 (MQ=255)

                            gCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCt                                      >  1:1820512/1‑71 (MQ=255)

                            gCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCt                                      >  1:647471/1‑71 (MQ=255)

                            gCCTGATGTTCTTCGGTGCAGTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCt                                      >  1:1910285/1‑71 (MQ=255)

                                     tcttcGGTGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCa                               >  1:401726/1‑69 (MQ=255)

                                            tGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCg                                  <  1:812597/59‑1 (MQ=255)

                                            tGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCg                                  <  1:12916/59‑1 (MQ=255)

                                            tGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCg                                  <  1:1401104/59‑1 (MQ=255)

                                            tGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCg                                  <  1:2296240/59‑1 (MQ=255)

                                            tGCATTTGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCg                                  <  1:1513803/59‑1 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAg                                               >  1:60294/1‑42 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:1330301/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:1415766/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:3340886/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:3435525/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:4093977/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:3465930/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCACAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:3373797/1‑71 (MQ=255)

                                                tttGCCGATTTCCTGCATGTAGATCAGACCTTCACAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgt                  >  1:3368990/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCg                                        >  1:1674713/1‑47 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:1900104/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:795387/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:962170/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:2322418/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:2180978/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:3332772/1‑71 (MQ=255)

                                                  tGCCGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttc                >  1:2017636/1‑71 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:3028155/71‑1 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:3098626/71‑1 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:514122/71‑1 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:504418/71‑1 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:3956936/71‑1 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:1875950/71‑1 (MQ=255)

                                                     cGATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             <  1:1859080/71‑1 (MQ=255)

                                                      gATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTTCCGTCAAAGGCCAGTTTgttcggtt            >  1:394308/1‑71 (MQ=255)

                                                      gATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggtt            >  1:3311937/1‑71 (MQ=255)

                                                      gATTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttcggt             >  1:3250360/1‑70 (MQ=255)

                                                       aTTTCCTGCTTGTAGATCAGACCTTCCCAGGCCAGATa                                            <  1:1306178/38‑1 (MQ=37)

                                                       aTTTCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTgttctgt             <  1:3778011/69‑1 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:1933514/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:3736750/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:3705944/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:586976/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:351928/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:3517075/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:768288/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:1916514/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:2921578/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:2789570/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGa         >  1:2523790/1‑70 (MQ=255)

                                                          tCCTGCATGTAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTAGGTTCGa         >  1:2416346/1‑70 (MQ=255)

                                                                atgtaGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGATAACCTc  <  1:343007/71‑1 (MQ=255)

GCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGATAACCTC  >  W3110S.gb/3100514‑3100648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`