BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,125,146	C→T	L11L (CTG→CTA)	glcE ←	glycolate oxidase FAD binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,125,146	0	C	T	100.0%	105.8 / NA	34	L11L (CTG→CTA)	glcE	glycolate oxidase FAD binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/10); total (24/10)

TTGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTA  >  W3110S.gb/3125087‑3125206

ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg                                                     <  1:1025222/69‑1 (MQ=255)

ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg                                                     <  1:681002/69‑1 (MQ=255)

ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg                                                     <  1:2281212/69‑1 (MQ=255)

ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg                                                     <  1:2757991/69‑1 (MQ=255)

   ctgcCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt                                                 <  1:79559/70‑1 (MQ=255)

   ctgcCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt                                                 <  1:2150714/70‑1 (MQ=255)

        cTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt                                                 <  1:1337382/65‑1 (MQ=255)

                     ggcgTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt                                                 <  1:2157407/52‑1 (MQ=255)

                           ttgtCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa                               >  1:2764027/4‑64 (MQ=255)

                           ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa                               >  1:3192043/1‑64 (MQ=255)

                           ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa                               >  1:3338095/1‑64 (MQ=255)

                           ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa                               >  1:3351283/1‑64 (MQ=255)

                           ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa                               >  1:2652375/1‑64 (MQ=255)

                              tCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCAGACTCGCGTAGCATCAGAAACg                      <  1:1900659/70‑1 (MQ=255)

                              tCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACg                      <  1:619463/70‑1 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTcg                                      >  1:3158727/1‑52 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:3406703/1‑71 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:474284/1‑71 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:793714/1‑71 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:3036567/1‑71 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:2604519/1‑71 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:1902142/1‑71 (MQ=255)

                                gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                   >  1:1688919/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTTGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:2376556/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:2948719/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:2781903/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:3377952/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:3766390/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:4005955/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:4102186/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:479162/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:1876446/1‑71 (MQ=255)

                                         cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg          >  1:979176/1‑71 (MQ=255)

                                                  cTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTa  >  1:1964379/1‑70 (MQ=255)

TTGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTA  >  W3110S.gb/3125087‑3125206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`