Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,135,166	A→G	intergenic (‑139/+153)	pitB ← / ← gss	phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,135,166	0	A	G	100.0%	273.5 / NA	75	intergenic (‑139/+153)	pitB/gss	phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/37);  total (38/37)

AAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAA  >  W3110S.gb/3135099‑3135228                                                                    |                                                               aaaTATCATTTTTTAACGATAAAAAGAGGGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGAc                                                              <  1:3967341/70‑1 (MQ=255)      tCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt                                                           >  1:1601051/1‑68 (MQ=255)            tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaata                                                   <  1:3517229/70‑1 (MQ=255)                aCGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa                                              <  1:1125718/71‑1 (MQ=255)                aCGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa                                              <  1:3854067/71‑1 (MQ=255)                aCGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa                                              <  1:1368873/71‑1 (MQ=255)                aCGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa                                              <  1:1947109/71‑1 (MQ=255)                aCGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACC‑TTAGAAATAATCaa                                              <  1:894965/70‑1 (MQ=255)                aCGATAAAAAGAGTGTTATTTCTTGTGTATAAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa                                              <  1:806815/71‑1 (MQ=255)                aCGATAAAAAGAGTGCTATTTCTTGTGTATCAGACAATAAGCTTTCATTCAAGACCTTTAGAAATAATCaa                                              <  1:3832542/71‑1 (MQ=255)                           agTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTAt                                   <  1:3044929/71‑1 (MQ=255)                               ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa                               >  1:2158709/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACGATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:3098385/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:3860627/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:1110754/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:1118895/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:404219/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:1354523/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:963878/1‑71 (MQ=255)                                 aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:961844/1‑71 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCCAAACACAGGTATTCCACTGGTTaa                       <  1:3897548/70‑1 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGTTTaa                       >  1:2647768/1‑70 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       >  1:1612859/1‑70 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       >  1:3055829/1‑70 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       <  1:4041097/70‑1 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       <  1:3853148/70‑1 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       >  1:793525/1‑70 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       <  1:2501554/70‑1 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       <  1:1506589/70‑1 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       >  1:223737/1‑70 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       <  1:1578496/70‑1 (MQ=255)                                        gtgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa                       <  1:2147247/70‑1 (MQ=255)                                           tATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatata                   <  1:417604/71‑1 (MQ=255)                                           tATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatata                   <  1:88028/71‑1 (MQ=255)                                           tATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatata                   <  1:3326910/71‑1 (MQ=255)                                           tATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAata                     <  1:3158558/69‑1 (MQ=255)                                           tATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAata                     <  1:668476/69‑1 (MQ=255)                                                gACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt                 <  1:3085470/68‑1 (MQ=255)                                                gACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt                 <  1:2676016/68‑1 (MQ=255)                                                gACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt                 <  1:2568272/68‑1 (MQ=255)                                                gACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt                 <  1:2334443/68‑1 (MQ=255)                                                gACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt                 <  1:1628836/68‑1 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:494473/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3115060/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3830533/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3499132/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3294868/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3448184/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:182779/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3266064/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:2520524/1‑68 (MQ=255)                                                  cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTca               >  1:3115875/1‑68 (MQ=255)                                                     tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc                                 <  1:4094350/47‑1 (MQ=255)                                                     tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc                                 <  1:2969103/47‑1 (MQ=255)                                                     tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc                                 <  1:3032408/47‑1 (MQ=255)                                                       aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa                               >  1:985112/1‑47 (MQ=255)                                                       aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa                               >  1:2476965/1‑47 (MQ=255)                                                       aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa                               >  1:2508565/1‑47 (MQ=255)                                                       aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa                               >  1:3022806/1‑47 (MQ=255)                                                       aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa                               >  1:3627746/1‑47 (MQ=255)                                                          attcattcAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTTATATATTCACATatgaaatg    <  1:3115586/71‑1 (MQ=255)                                                          attcattcAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg    <  1:3946089/71‑1 (MQ=255)                                                          attcattcAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg    <  1:1077558/71‑1 (MQ=255)                                                          attcattcAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg    <  1:2623726/71‑1 (MQ=255)                                                          attcattcAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg    <  1:684537/71‑1 (MQ=255)                                                          attcattcAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg    <  1:3749363/71‑1 (MQ=255)                                                           ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt                 <  1:3614469/57‑1 (MQ=255)                                                            tcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatg         >  1:2765005/1‑64 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt                             >  1:54606/1‑43 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatgaa  >  1:3005947/1‑70 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatgaa  >  1:3152813/1‑70 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatgaa  >  1:2509962/1‑70 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatgaa  >  1:133581/1‑70 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatgaa  >  1:3576946/1‑70 (MQ=255)                                                             cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAATCACATatgaaatgaa  >  1:1186730/1‑70 (MQ=255)                                                                    |                                                               AAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAA  >  W3110S.gb/3135099‑3135228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑