| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,186,703 | T→C | F216F (TTT→TTC) | yqiG → | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,186,703 | 0 | T | C | 76.7% | 116.9 / 33.5 | 61 | F216F (TTT→TTC) | yqiG | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base T (8/6); new base C (21/25); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAC > W3110S.gb/3186638‑3186766 | cGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCcgcg < 1:400561/71‑1 (MQ=255) cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:2868943/1‑71 (MQ=255) cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:1068512/1‑71 (MQ=255) tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCcgcg < 1:1863192/66‑1 (MQ=255) tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCcgcg < 1:1679566/66‑1 (MQ=255) tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCcgcg < 1:916947/66‑1 (MQ=255) tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCcgcg < 1:1263814/66‑1 (MQ=255) aTTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGtattta < 1:3457350/70‑1 (MQ=255) aTTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGta > 1:1797393/1‑66 (MQ=255) ttACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGTCCAACCTGGATTTCCCGCGtattta < 1:2462304/69‑1 (MQ=255) tACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTatttattt > 1:262700/1‑71 (MQ=255) aCCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGtatttatt > 1:768005/1‑69 (MQ=255) aCCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGtatttatt > 1:2292120/1‑69 (MQ=255) aCCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGtatttatt > 1:3301384/1‑69 (MQ=255) aCCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGtatttatt > 1:1195366/1‑69 (MQ=255) ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTg < 1:3971743/70‑1 (MQ=255) ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGt < 1:435232/71‑1 (MQ=255) ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGt < 1:118076/71‑1 (MQ=255) ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGt < 1:3287714/71‑1 (MQ=255) cAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTg < 1:475698/69‑1 (MQ=255) gTACGATCAGTCATTTTACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTg < 1:3319342/67‑1 (MQ=255) cGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGcc > 1:444731/1‑71 (MQ=255) cGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGcc > 1:2104628/1‑71 (MQ=255) cGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGcc > 1:1219443/1‑71 (MQ=255) tCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCaa > 1:225768/1‑71 (MQ=255) tCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCaa > 1:938340/1‑71 (MQ=255) tCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGccc > 1:709888/1‑65 (MQ=255) tCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGccc > 1:1430498/1‑65 (MQ=255) aTTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:267031/1‑49 (MQ=255) aTTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:1006625/1‑49 (MQ=255) aTTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:313412/1‑49 (MQ=255) aTTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:3484828/1‑49 (MQ=255) aTTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:3228019/1‑49 (MQ=255) aTTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt > 1:713633/1‑49 (MQ=255) gaaaaGCCAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGt < 1:491758/43‑1 (MQ=39) cAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCATCAATGAATGCAAaactaac > 1:1544397/1‑71 (MQ=255) cAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactaac > 1:3712872/1‑71 (MQ=255) cAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactaac < 1:1021861/71‑1 (MQ=255) cAGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactaac > 1:1755735/1‑71 (MQ=255) cAGGCGACCAACCTGGATTTACCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactaac > 1:3327853/1‑71 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:1094937/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:1430027/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:64203/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:561972/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:1489244/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:2877944/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:314864/68‑1 (MQ=255) aGGCGACCAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta < 1:3482452/68‑1 (MQ=255) cAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt > 1:2258079/1‑70 (MQ=255) cAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt > 1:428443/1‑70 (MQ=255) cAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt > 1:1528742/1‑70 (MQ=255) cAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt > 1:344368/1‑70 (MQ=255) cAACCTGGATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt > 1:642024/1‑70 (MQ=255) cTGGATTTCCCGCGTATTTATTTGTTTCGCGCAATCCCAGCAATGAATGCAAaacta < 1:1440961/57‑1 (MQ=255) gATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCACTAc < 1:1209581/69‑1 (MQ=255) gATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc < 1:1785409/69‑1 (MQ=255) gATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc < 1:3364687/69‑1 (MQ=255) gATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc < 1:3360002/69‑1 (MQ=255) gATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc < 1:1219324/69‑1 (MQ=255) gATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc < 1:3343112/69‑1 (MQ=255) gATTTCCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc < 1:1577497/69‑1 (MQ=255) | CGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAC > W3110S.gb/3186638‑3186766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |