| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,194,164 | T→C | T416A (ACG→GCG) | rfaE ← | fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,194,164 | 0 | T | C | 92.9% | 87.0 / ‑1.0 | 28 | T416A (ACG→GCG) | rfaE | fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (12/14); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCA > W3110S.gb/3194098‑3194220 | ctGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTc < 1:1460846/70‑1 (MQ=255)ctGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTc < 1:908651/70‑1 (MQ=255) gTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAAc < 1:859770/70‑1 (MQ=255) tCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:2677296/71‑1 (MQ=255) tCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:2500817/71‑1 (MQ=255) tCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:1543665/71‑1 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt < 1:1047901/71‑1 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt < 1:3555518/71‑1 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt < 1:3691672/71‑1 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt < 1:3185800/71‑1 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt < 1:2365730/71‑1 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:2429472/1‑70 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:2975840/1‑70 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:1271370/1‑70 (MQ=255) tGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTcc < 1:694333/37‑1 (MQ=255) gCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCctc > 1:3608792/1‑37 (MQ=255) gCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTcc < 1:1296219/68‑1 (MQ=255) gCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc < 1:1517495/67‑1 (MQ=255) gCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc < 1:3071440/67‑1 (MQ=255) gATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAAc < 1:1367848/40‑1 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTgcga > 1:1113335/1‑57 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:3232308/1‑70 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:3721054/1‑70 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:626160/1‑70 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:2949650/1‑70 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:83889/1‑70 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:1848993/1‑70 (MQ=255) aaGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAAACa > 1:1541543/1‑70 (MQ=255) | CTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCA > W3110S.gb/3194098‑3194220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |