| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,272,678 | A→T | S245S (TCT→TCA) | garL ← | alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,272,678 | 0 | A | T | 80.4% | 115.2 / 24.2 | 51 | S245S (TCT→TCA) | garL | alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/9); new base T (27/14); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.91e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGC > W3110S.gb/3272614‑3272744 | tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCGAGTTTCTGAGTGGCa < 1:319928/65‑1 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCa < 1:4130610/65‑1 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCa < 1:372860/65‑1 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCa < 1:2285462/65‑1 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCa < 1:2207372/65‑1 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCg > 1:3647787/1‑70 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCg > 1:3717049/1‑70 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCg > 1:2810942/1‑70 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCg > 1:2406224/1‑70 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCg > 1:3568491/1‑70 (MQ=255)tcaatcaTCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCg > 1:4098283/1‑70 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:3057071/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:1762401/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:1799613/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:2018771/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:2804488/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:153139/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:2631689/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGa < 1:2596106/71‑1 (MQ=255) tcatcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGAAAGa < 1:1802477/71‑1 (MQ=255) ctctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGcc > 1:3677649/1‑71 (MQ=255) ctctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGcc > 1:2912756/1‑71 (MQ=255) ctctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGcc > 1:3408303/1‑71 (MQ=255) ctctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGcc > 1:3261448/1‑71 (MQ=255) tctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCgg < 1:1520918/62‑1 (MQ=255) tctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCgg < 1:2029769/62‑1 (MQ=255) tctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGaa < 1:2562996/62‑1 (MQ=255) tctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGaa < 1:136605/62‑1 (MQ=255) tctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGaa < 1:2803934/62‑1 (MQ=255) ttgttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCgag < 1:162113/69‑1 (MQ=255) ttgttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCgag < 1:3921659/69‑1 (MQ=255) ttgttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGa > 1:2442771/1‑62 (MQ=255) tggtTATTTTTTAAAGGTATCAGCCAGTTTCT‑AGTGGCTGAGCGGAAGa < 1:1423239/49‑1 (MQ=38) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCgg > 1:3266903/1‑42 (MQ=255) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGAt > 1:3396619/1‑55 (MQ=255) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGAt > 1:2338139/1‑55 (MQ=255) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGAt > 1:2703883/1‑55 (MQ=255) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGAt > 1:2255646/1‑55 (MQ=255) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGAt > 1:1262514/1‑55 (MQ=255) tATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGAt > 1:764196/1‑55 (MQ=255) tATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGccc > 1:2164486/1‑71 (MQ=255) tATCAGCCAGTTTCTGAGTGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGACACAAACGt > 1:822857/1‑64 (MQ=255) aGTGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAacgac > 1:834164/1‑71 (MQ=255) aGTGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAacgac > 1:3766813/1‑71 (MQ=255) aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAac < 1:2965337/68‑1 (MQ=255) aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAac < 1:2313634/68‑1 (MQ=255) aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAac < 1:1580435/68‑1 (MQ=255) aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAac < 1:1215682/68‑1 (MQ=255) aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAac < 1:121505/68‑1 (MQ=255) aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAac < 1:1006016/68‑1 (MQ=255) tGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAacgacg > 1:2599461/1‑70 (MQ=255) tGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAacgacg > 1:4098592/1‑70 (MQ=255) tGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGAcgc > 1:320285/1‑71 (MQ=255) tGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGAcgc > 1:3783603/1‑71 (MQ=255) tGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGAcgc > 1:1617272/1‑71 (MQ=255) tGGCTGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGAcgc > 1:1268692/1‑71 (MQ=255) | TCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGC > W3110S.gb/3272614‑3272744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |