| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,358,271 | A→G | K1262R (AAA→AGA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,358,271 | 0 | A | G | 77.8% | 71.6 / 16.8 | 36 | K1262R (AAA→AGA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/7); new base G (19/9); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
TGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGCC > W3110S.gb/3358203‑3358338 | tGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCagaa > 1:710324/1‑71 (MQ=255)tGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCagaa > 1:2150003/1‑71 (MQ=255) tAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCt > 1:624483/1‑71 (MQ=255) tAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCt > 1:2119682/1‑71 (MQ=255) tAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCt > 1:3447076/1‑71 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:2776675/69‑1 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:2606004/69‑1 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:1712940/69‑1 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:1863530/69‑1 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:552409/69‑1 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:3211574/69‑1 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc < 1:2390714/69‑1 (MQ=255) ctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCagaa < 1:885570/43‑1 (MQ=255) ctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCagaa < 1:3897707/43‑1 (MQ=255) tgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACaccgac > 1:2427644/1‑71 (MQ=255) tgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACaccgac > 1:3059178/1‑71 (MQ=255) tgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACaccga > 1:3186002/1‑70 (MQ=255) aCCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATAtt < 1:871941/46‑1 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGc < 1:988202/39‑1 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt < 1:2847299/70‑1 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt < 1:2291749/70‑1 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt < 1:3944162/70‑1 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:2450555/1‑71 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg < 1:1120319/71‑1 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:1565361/1‑71 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:1940695/1‑71 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:1945380/1‑71 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg < 1:485348/71‑1 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:4053817/1‑71 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:3235526/1‑71 (MQ=255) tGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAg > 1:2391801/1‑71 (MQ=255) aGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAgg > 1:460759/1‑70 (MQ=255) gagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt < 1:1961567/59‑1 (MQ=255) gagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt < 1:3410498/59‑1 (MQ=255) gCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGcc > 1:3913189/1‑71 (MQ=255) gCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGcc > 1:3728614/1‑71 (MQ=255) gCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGcc > 1:3622434/1‑71 (MQ=255) gCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGcc > 1:972158/1‑71 (MQ=255) gCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGcc > 1:1114569/1‑71 (MQ=255) gCAGAACCTACTGGTTCGATATTCGCAACACCGACCGt > 1:1255111/1‑38 (MQ=38) | TGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGCC > W3110S.gb/3358203‑3358338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |