| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,363,356 | G→A | S232N (AGC→AAC) | yhcD → | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,363,356 | 0 | G | A | 88.3% | 265.9 / 22.6 | 94 | S232N (AGC→AAC) | yhcD | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (11/0); new base A (26/57); total (37/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
ACGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCATTGCAAACCAGC > W3110S.gb/3363297‑3363420 | aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTTGAAg < 1:489141/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:3777406/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:2253428/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:2058155/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:2053307/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:1519362/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:1482206/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:1399397/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:3148614/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:1131152/71‑1 (MQ=255)aCGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg < 1:3768342/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:3174290/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:2583056/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:1994145/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:2583306/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:2837631/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:3757946/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:2968644/71‑1 (MQ=255) tACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAg < 1:4121644/71‑1 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:1575041/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:2218931/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:1101242/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:2977759/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:3455660/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:1858460/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:183501/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:468266/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:594818/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:275712/1‑70 (MQ=255) ccTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGc > 1:2855335/1‑70 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:2705174/1‑56 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:3697611/1‑56 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:46691/1‑56 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:937656/1‑56 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:3152605/1‑56 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:8549/1‑56 (MQ=255) tCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAg > 1:1737123/1‑56 (MQ=255) cATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTgg > 1:3001594/1‑41 (MQ=255) cATGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTgg > 1:3612340/1‑41 (MQ=255) aTGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACg > 1:686869/1‑58 (MQ=255) aTGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACg > 1:1668290/1‑58 (MQ=255) aTGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACg > 1:718918/1‑58 (MQ=255) aTGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGc < 1:1426275/71‑1 (MQ=255) aTGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGc < 1:731692/71‑1 (MQ=255) aTGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGc < 1:1228249/71‑1 (MQ=255) ttcGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCa < 1:3273233/69‑1 (MQ=255) tGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCa < 1:2740758/71‑1 (MQ=255) tGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCa < 1:2735967/71‑1 (MQ=255) tGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCa < 1:1805445/71‑1 (MQ=255) tGCGAGCAGGTGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCa < 1:3777819/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:4023841/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:3086695/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:2342708/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:3824159/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:2531903/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:2793470/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:2788871/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:2783106/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:1781120/71‑1 (MQ=255) tGAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg < 1:591946/71‑1 (MQ=255) gAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg > 1:1287224/1‑70 (MQ=255) gAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg > 1:3154164/1‑70 (MQ=255) gAAGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACg > 1:1793773/1‑70 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1426611/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1202398/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1948419/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1203283/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:2691861/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:2740113/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1358991/61‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAt < 1:3865157/71‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAt < 1:3422231/71‑1 (MQ=255) aGTTAACCTCAATAATAACGTGTTGAAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1089527/61‑1 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:3801585/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:1030814/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:858552/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:1193626/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:121609/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:2677011/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:1318219/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:2667976/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:496622/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCAtt > 1:1969054/1‑71 (MQ=255) gTTAACCTCAATAATAGCGTGTAGGAAGGAGCCAGTATTTACGGTATCGAAATCGCCCCgg > 1:3549018/1‑61 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:3734301/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1491269/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:606182/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:1783927/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:474289/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:2884986/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:2909502/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:321940/51‑1 (MQ=255) aataatAACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCg < 1:3985385/51‑1 (MQ=255) ataataACGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCATTGCAAACCAGc < 1:970987/71‑1 (MQ=255) | ACGTACCTTTACAGATCTTAAAACACTCATGCGAGCAGGTGAAGTTAACCTCAATAATAGCGTGTTGGAAGGAGCCAGTATTTACGGTATCGAAATCGCACCGGACAACGCATTGCAAACCAGC > W3110S.gb/3363297‑3363420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |