| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,400,733 | A→G | I70I (ATT→ATC) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,400,733 | 0 | A | G | 92.7% | 128.9 / 0.8 | 41 | I70I (ATT→ATC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base G (22/16); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCG > W3110S.gb/3400665‑3400797 | tGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGat < 1:1365950/71‑1 (MQ=255)tGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGat < 1:2838337/71‑1 (MQ=255)tGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGat < 1:4065547/71‑1 (MQ=255)tGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGat < 1:401277/71‑1 (MQ=255) gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTg < 1:270402/70‑1 (MQ=255) gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTg < 1:2267851/70‑1 (MQ=255) gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTg < 1:337770/70‑1 (MQ=255) gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTg < 1:1683523/70‑1 (MQ=255) gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTg < 1:1367116/70‑1 (MQ=255) gCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGccc > 1:3958884/1‑71 (MQ=255) ttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCg > 1:1877269/1‑70 (MQ=255) aCGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCa > 1:2193382/1‑70 (MQ=255) aCGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAg > 1:3981581/1‑71 (MQ=255) aCGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAg > 1:250085/1‑71 (MQ=255) gATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGt < 1:3370461/71‑1 (MQ=255) gATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGt < 1:2914335/71‑1 (MQ=255) gATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGt < 1:2130496/71‑1 (MQ=255) gATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGt < 1:3669317/71‑1 (MQ=255) gATAACGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGt < 1:3998081/71‑1 (MQ=255) aCGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATg < 1:2897348/71‑1 (MQ=255) aCGCCGTCTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATg < 1:1858897/71‑1 (MQ=255) ccGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGAcc < 1:96933/71‑1 (MQ=255) ccGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGAcc < 1:333172/71‑1 (MQ=255) ccGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGAcc < 1:1754267/71‑1 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCtt > 1:3553527/1‑55 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTAc > 1:1891993/1‑70 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3305929/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:83111/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:1449405/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:1792794/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:1793658/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3407205/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3152914/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3374507/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3214096/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3257258/1‑71 (MQ=255) cTTTCATTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:3276787/1‑71 (MQ=255) cTTTCAGTGGGCGAATGGCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACt > 1:526286/1‑71 (MQ=255) ggCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTcgcg > 1:1057167/1‑43 (MQ=255) ggCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCg > 1:2390394/1‑71 (MQ=255) ggCAGCGATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCg > 1:817598/1‑71 (MQ=255) | TGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCG > W3110S.gb/3400665‑3400797 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |