Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,447,843	A→G	V1163V (GTT→GTC)	rpoC ←	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,447,843	0	A	G	100.0%	226.1 / NA	62	V1163V (GTT→GTC)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/34);  total (28/34)

ACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGG  >  W3110S.gb/3447776‑3447903                                                                    |                                                             aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:3489204/1‑70 (MQ=255) aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:1417126/1‑70 (MQ=255) aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:2748077/1‑70 (MQ=255) aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:1639302/1‑70 (MQ=255) aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:3265362/1‑70 (MQ=255) aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:624789/1‑70 (MQ=255) aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc                                                            >  1:3434406/1‑70 (MQ=255)    gATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATa                                                        >  1:1965081/1‑71 (MQ=255)                       ggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgta                                     <  1:46713/71‑3 (MQ=255)                            tAACCAGACGACGTTTGCCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:943004/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:2370794/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:2412602/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:2354908/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:1958780/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:679121/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:1579534/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:3317027/65‑1 (MQ=255)                            tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:825212/65‑1 (MQ=255)                             aaCCAGACGACGTTTACGTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:1047635/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGCCGATACCGCTGATTTCAGCCAGGATTGcc                                >  1:3535840/1‑70 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:313286/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:3406580/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:2661528/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:3330108/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:3400969/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:2085234/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:560064/1‑71 (MQ=255)                             aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg                               >  1:751329/1‑71 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:3593652/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:3889280/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:2545225/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:2252493/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:1195489/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:617147/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:3217189/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:1658917/71‑1 (MQ=255)                                   acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:1411745/71‑1 (MQ=255)                                      acgTTTAGCTTTGGTTTCTTTAGCGAAGGAGACGATACCGCTGATTTCAGCCAgg                                      <  1:4071012/55‑1 (MQ=255)                                           tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga                 >  1:2375433/1‑71 (MQ=255)                                               tttGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGa                                     >  1:2078199/1‑47 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg                                       >  1:3480970/1‑43 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg                                       >  1:946530/1‑43 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg                                       >  1:1148283/1‑43 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg                                       >  1:274277/1‑43 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg                                       >  1:2390342/1‑43 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg                                       >  1:1828838/1‑43 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg             <  1:3510285/69‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg             <  1:2287266/69‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg             <  1:1957149/69‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg             <  1:1817255/69‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc            <  1:2641558/70‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc            <  1:1353769/70‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc            <  1:1452786/70‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc            <  1:2359504/70‑1 (MQ=255)                                                 tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc            <  1:1631987/70‑1 (MQ=255)                                                     ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCg        >  1:2208611/1‑70 (MQ=255)                                                     ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCg        >  1:3268019/1‑70 (MQ=255)                                                          tACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGct                            <  1:2422691/45‑1 (MQ=255)                                                          tACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGct                            <  1:1339128/45‑1 (MQ=255)                                                           aCCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAgg  <  1:3496965/70‑1 (MQ=255)                                                            ccGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc            <  1:2876473/59‑1 (MQ=255)                                                             cGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt                         <  1:669025/45‑1 (MQ=255)                                                                    |                                                             ACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGG  >  W3110S.gb/3447776‑3447903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑