Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,573,836	A→G	V200A (GTG→GCG)	yihN ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,573,836	0	A	G	91.5%	145.0 / 3.8	47	V200A (GTG→GCG)	yihN	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base G (13/30);  total (13/34)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

TAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAA  >  W3110S.gb/3573774‑3573887                                                               |                                                    tAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAacg                                             <  1:3530592/71‑1 (MQ=255) tAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAacg                                             <  1:74865/71‑1 (MQ=255) tAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAacg                                             <  1:252901/71‑1 (MQ=255) tAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAacg                                             <  1:3739087/71‑1 (MQ=255)           tttCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGt                                       <  1:3655940/67‑1 (MQ=255)           tttCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGt                                       <  1:483209/67‑1 (MQ=255)           tttCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGt                                       <  1:1974342/67‑1 (MQ=255)           tttCAGCACCGAGGTCATACTGGGGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGt                                       <  1:1685187/67‑1 (MQ=255)                   ccGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGt                                       <  1:477273/59‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:3218215/71‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:3674561/71‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:235219/71‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:3728145/71‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:3223070/71‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:711331/71‑1 (MQ=255)                           aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTATCATTCAg                   <  1:4075222/71‑1 (MQ=255)                            tACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:668949/70‑1 (MQ=255)                                    tttttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaa          <  1:2432110/71‑1 (MQ=255)                                    tttttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaa          <  1:1235891/71‑1 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   >  1:1016165/1‑60 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:4039556/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:1220918/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:264390/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:2197952/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:2110531/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:1678405/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa        >  1:1995911/1‑71 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaa         >  1:1534184/1‑70 (MQ=255)                                      tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaa         >  1:748606/1‑70 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAAGGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:3840854/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:860500/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:3161118/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:748612/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:163088/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:659797/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:1749116/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:1884025/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:1999768/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:3942146/68‑1 (MQ=255)                                          gATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:3744167/68‑1 (MQ=255)                                           aTGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:741365/67‑1 (MQ=255)                                                   tcttctTTTTTCGACTCAACGGACGGTGCCTCTTCTTTGTCATTCAg                   <  1:285997/47‑1 (MQ=255)                                                       cttTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataat    <  1:3383471/58‑1 (MQ=255)                                                         tttttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa  >  1:42031/1‑58 (MQ=255)                                                         tttttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa  >  1:496929/1‑58 (MQ=255)                                                         tttttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa  >  1:3019146/1‑58 (MQ=255)                                                          ttttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaat       <  1:2999896/52‑1 (MQ=255)                                                               |                                                    TAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAA  >  W3110S.gb/3573774‑3573887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑