Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	480,905	C→T	G908E (GGG→GAG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	480,905	0	C	T	100.0%	167.8 / NA	52	G908E (GGG→GAG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/12);  total (40/12)

GTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAG  >  W3110S.gb/480850‑480972                                                        |                                                                    gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:1088484/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:3659218/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:3420965/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:3267027/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:820740/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:2289253/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:2172310/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:2021798/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:1870901/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:139490/1‑69 (MQ=255) gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc                                                        >  1:1395044/1‑69 (MQ=255)              gTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGgaga                                          >  1:1846182/1‑70 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:2459771/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:3694406/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:4115757/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:1102290/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:2485314/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:1840382/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:2060969/1‑71 (MQ=255)                   gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa                                    >  1:1970997/1‑71 (MQ=255)                    ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAAt                                   >  1:464214/1‑71 (MQ=255)                    ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAAt                                   >  1:1078406/1‑71 (MQ=255)                    ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAAt                                   >  1:855210/1‑71 (MQ=255)                      aCGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCg                                 <  1:2351701/71‑1 (MQ=255)                      aCGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCg                                 <  1:2356581/71‑1 (MQ=255)                       cGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCg                                 <  1:331860/70‑1 (MQ=255)                             gTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGct                          <  1:682819/71‑1 (MQ=255)                                gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct                        <  1:2640409/70‑1 (MQ=255)                                gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct                        <  1:2168787/70‑1 (MQ=255)                                gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct                        <  1:1222515/70‑1 (MQ=255)                                gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct                        <  1:820848/70‑1 (MQ=255)                                gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct                        <  1:19506/70‑1 (MQ=255)                                gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct                        <  1:1693871/70‑1 (MQ=255)                                      agcAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAg                 <  1:1236721/71‑1 (MQ=255)                                      agcAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAg                 <  1:3937031/71‑1 (MQ=255)                                               ccGATATCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:527776/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:804349/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:956342/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:4025191/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:3626084/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:2545043/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:2395450/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc           >  1:1515798/1‑68 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccg            >  1:3938339/1‑67 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccg            >  1:2021732/1‑67 (MQ=255)                                               ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgacgc           >  1:2060837/1‑68 (MQ=255)                                                     aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg  >  1:3777097/1‑71 (MQ=255)                                                     aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg  >  1:3741713/1‑71 (MQ=255)                                                     aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg  >  1:4109802/1‑71 (MQ=255)                                                     aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg  >  1:3365355/1‑71 (MQ=255)                                                     aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg  >  1:3361499/1‑71 (MQ=255)                                                     aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg  >  1:2701715/1‑71 (MQ=255)                                                        |                                                                    GTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAG  >  W3110S.gb/480850‑480972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑