| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,682,573 | G→A | L211L (CTG→TTG) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,682,573 | 0 | G | A | 80.0% | 67.2 / 14.7 | 35 | L211L (CTG→TTG) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/7); new base A (9/19); total (9/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01 | |||||||||||
ACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACA > W3110S.gb/3682520‑3682639 | aCGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAg < 1:778757/70‑1 (MQ=255)aCGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAg < 1:3929602/70‑1 (MQ=255)aCGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAg < 1:3440788/70‑1 (MQ=255)aCGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAg < 1:2935939/70‑1 (MQ=255) ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTc < 1:1610422/71‑1 (MQ=255) ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGGCAGGCAGTTc < 1:3715311/71‑1 (MQ=255) gCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGa < 1:3309132/71‑1 (MQ=255) ggTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAg > 1:719868/1‑71 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:1104287/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:768388/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:4065545/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:3612676/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:1191269/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:2359584/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:1511882/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAg > 1:2148487/1‑60 (MQ=255) agcagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:1084102/71‑1 (MQ=255) gcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATc < 1:2865737/71‑1 (MQ=255) gcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATc < 1:3076347/71‑1 (MQ=255) gcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATc < 1:1419059/71‑1 (MQ=255) gcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATc < 1:2161121/71‑1 (MQ=255) gcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATc < 1:1655147/71‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:244949/48‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:805525/48‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:362914/48‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:3516703/48‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:3360935/48‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:2666817/48‑1 (MQ=255) cGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTaaa < 1:2398848/48‑1 (MQ=255) ggCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGa < 1:3301040/71‑1 (MQ=255) cccAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca < 1:1703341/71‑1 (MQ=255) cccAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca < 1:1348639/71‑1 (MQ=255) cccAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca < 1:2929345/71‑1 (MQ=255) cccAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca < 1:904796/71‑1 (MQ=255) cccAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGGCGGaca < 1:9223/71‑1 (MQ=255) | ACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACA > W3110S.gb/3682520‑3682639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |