Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	515,934	C→T	A43V (GCG→GTG)	ybbM →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	515,934	0	C	T	100.0%	157.4 / NA	52	A43V (GCG→GTG)	ybbM	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/41);  total (11/41)

TTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATG  >  W3110S.gb/515873‑515993                                                              |                                                            ttAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt                                                      >  1:3950006/1‑69 (MQ=255)        gCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat                                              >  1:2786847/1‑70 (MQ=255)              aaGAAAAACTGGCGCTGGATAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc                                       >  1:1774181/1‑71 (MQ=255)              aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc                                       >  1:2731123/1‑71 (MQ=255)              aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc                                       >  1:4022952/1‑71 (MQ=255)              aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc                                       >  1:3893150/1‑71 (MQ=255)              aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc                                       >  1:2697507/1‑71 (MQ=255)              aaGAAAAACTGGAGCTGGAGAAAGATATTCTCTGTAGCGTCGGGCGAGTGATaa                                                        >  1:834678/1‑54 (MQ=38)               aGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCg                                      <  1:1889187/71‑1 (MQ=255)                  aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCgg                                     >  1:824633/1‑69 (MQ=255)                  aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc                                    >  1:3146924/1‑70 (MQ=255)                      cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTAtgt                               >  1:2355177/1‑71 (MQ=255)                         gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc                             <  1:801639/70‑1 (MQ=255)                         gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc                             <  1:2243346/70‑1 (MQ=255)                         gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc                             <  1:1138507/70‑1 (MQ=255)                         gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc                             <  1:1625892/70‑1 (MQ=255)                         gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc                             <  1:271669/70‑1 (MQ=255)                          cgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc                             <  1:4052089/69‑1 (MQ=255)                                 gaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGtata                    <  1:2119024/71‑1 (MQ=255)                                 gaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGtata                    <  1:4117372/71‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:4003382/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:3705325/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:3419525/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:3199102/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:3180525/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:1659623/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:1682133/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:2289558/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:1001035/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt                                             <  1:2654878/45‑1 (MQ=255)                                  aaaGATATTCTCTGGAGCGTCCGGCGAGTGATAATTCAGCTGatt                                             <  1:2569341/45‑1 (MQ=255)                                     gATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCg                                      <  1:616316/49‑1 (MQ=255)                                         ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta                      <  1:3303602/61‑1 (MQ=255)                                         ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta                      <  1:3464288/61‑1 (MQ=255)                                         ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta                      <  1:3743394/61‑1 (MQ=255)                                         ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta                      <  1:1211630/61‑1 (MQ=255)                                         ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta                      <  1:1908047/61‑1 (MQ=255)                                         ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta                      <  1:4014246/61‑1 (MQ=255)                                                gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat      <  1:377964/70‑1 (MQ=255)                                                gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat      <  1:2811560/70‑1 (MQ=255)                                                gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat      <  1:3059707/70‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:2935628/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:492087/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:827676/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:3870062/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:357490/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:2770350/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:2365155/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:1919344/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:1405050/71‑1 (MQ=255)                                                   cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:1066784/71‑1 (MQ=255)                                                       gggCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:417657/67‑1 (MQ=255)                                                         gcgagtgaTAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg  <  1:1965402/65‑1 (MQ=255)                                                              |                                                            TTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATG  >  W3110S.gb/515873‑515993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑