| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,066,786 | C→T | intergenic (+144/‑129) | asd → / → glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,066,786 | 0 | C | T | 85.9% | 378.0 / 47.4 | 142 | intergenic (+144/‑129) | asd/glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base C (13/7); new base T (61/61); total (74/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 142 total aligned reads displayed.
GATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCAAAAAAA > W3110S.gb/4066718‑4066851 | gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCt > 1:682416/1‑71 (MQ=255)gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCt > 1:1202609/1‑71 (MQ=255)gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCt > 1:2973426/1‑71 (MQ=255)gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCt > 1:3113677/1‑71 (MQ=255)gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCt > 1:332080/1‑71 (MQ=255)gATTTACCGGCAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATTAGTATTCACAGTCt > 1:3214382/1‑71 (MQ=255) aTTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCt < 1:2647698/70‑1 (MQ=255) aGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTg < 1:2880241/71‑1 (MQ=255) aGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTg < 1:3878978/71‑1 (MQ=255) aGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTg < 1:1637820/71‑1 (MQ=255) aaaTAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGga > 1:2373709/1‑71 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGGGGacgacg < 1:3007634/71‑1 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg < 1:2237919/71‑1 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg < 1:4069754/71‑1 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg < 1:2380979/71‑1 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg < 1:1724498/71‑1 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg < 1:2575386/71‑1 (MQ=255) gagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg < 1:2580155/71‑1 (MQ=255) ttGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagag < 1:2169310/71‑1 (MQ=255) ttGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagag < 1:206893/71‑1 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag > 1:3577727/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag > 1:204586/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag > 1:3764990/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag > 1:3683394/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag > 1:3394469/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:454489/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:271778/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:654317/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:956102/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1394075/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1436833/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1808449/1‑71 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTAACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:3282357/1‑71 (MQ=255) gCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:2420707/70‑1 (MQ=255) tttAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:3214522/63‑1 (MQ=255) tttAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:2019130/63‑1 (MQ=255) tttAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:31483/63‑1 (MQ=255) tttAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:2894044/63‑1 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGa > 1:2088139/1‑63 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca > 1:332557/1‑69 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca > 1:3725581/1‑69 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca > 1:1700366/1‑69 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca > 1:2446337/1‑69 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:1025172/69‑1 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:2545701/69‑1 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:2455393/69‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAgg < 1:3829155/59‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:512286/67‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:3478776/67‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:1469248/67‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:1482645/67‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:149731/67‑1 (MQ=255) gggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:1900139/67‑1 (MQ=255) ggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGCCGCAGAGAGGATGCACAGAGtgc > 1:2226733/1‑71 (MQ=255) ggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:3534013/1‑71 (MQ=255) ggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:777308/1‑71 (MQ=255) ggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:2032348/1‑71 (MQ=255) ggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:3309367/1‑71 (MQ=255) ggTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:3021217/1‑71 (MQ=255) gTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGga > 1:1169223/1‑44 (MQ=255) gTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCaga > 1:1694092/1‑53 (MQ=255) gTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCaga > 1:466128/1‑53 (MQ=255) gTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCaga > 1:4033156/1‑53 (MQ=255) gTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCaga > 1:3383619/1‑53 (MQ=255) gTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCag > 1:3861252/1‑52 (MQ=255) gTGGCTGAATAAATGAGTATTCACAGTCTTACATGAAGTGAGGACGACGCaga > 1:1142029/1‑53 (MQ=255) gCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgctgc > 1:1211912/1‑70 (MQ=255) gCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg > 1:3984468/1‑71 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:1772909/57‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:351938/57‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:2314936/57‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:426496/57‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:404453/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:3905788/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:115879/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:2327713/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:2818241/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:2561930/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:3463547/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:3411710/70‑1 (MQ=255) aaTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg < 1:899526/70‑1 (MQ=255) tACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACaga > 1:485258/1‑57 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAg < 1:3624506/71‑1 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAg < 1:3144831/71‑1 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAg < 1:2277950/71‑1 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAg < 1:66546/71‑1 (MQ=255) cATGAGTATTCACAGCCTTACCTGAAGTGTGGACGACGCAGAGAGGATGcaca < 1:835838/53‑1 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGAt > 1:1198493/1‑41 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca > 1:3249988/1‑46 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaa > 1:3961638/1‑70 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaa > 1:558202/1‑70 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaa > 1:3052868/1‑70 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaa > 1:215828/1‑70 (MQ=255) aTTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaa > 1:1037592/1‑70 (MQ=255) cacaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaaaa > 1:1646334/1‑71 (MQ=255) cacaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaaaa > 1:3074222/1‑71 (MQ=255) acaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGt < 1:1517923/46‑1 (MQ=255) acaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGt < 1:815666/46‑1 (MQ=255) acaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGt < 1:1498759/46‑1 (MQ=255) acaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGt < 1:1861719/46‑1 (MQ=255) | GATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCAAAAAAA > W3110S.gb/4066718‑4066851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |