BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,103,301	A→G	R102R (CGT→CGC)	greB ←	transcription elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,103,301	0	A	G	100.0%	222.4 / NA	62	R102R (CGT→CGC)	greB	transcription elongation factor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/26); total (36/26)

GGGGAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAACGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTG  >  W3110S.gb/4103239‑4103366

ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt                                                           <  1:3680535/71‑1 (MQ=255)

ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt                                                           >  1:1091496/1‑71 (MQ=255)

ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt                                                           >  1:3844804/1‑71 (MQ=255)

ggggAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGt                                                           <  1:4064224/71‑1 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCAc                                                        >  1:921828/1‑68 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:4137715/1‑70 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:3040676/1‑70 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:2814685/1‑70 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:2705810/1‑70 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:1084124/1‑70 (MQ=255)

      tCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:1210916/1‑70 (MQ=255)

              gaTGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATcgtcgt                                             <  1:2523430/71‑1 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCa                                                         >  1:3011013/1‑53 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:12472/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:3947188/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:3114584/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:3996126/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:2871795/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:1624322/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:4001097/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:2604922/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:2445962/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:2184004/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCAtcgtcg                                              >  1:1884332/1‑64 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGtttt                                          >  1:3962504/1‑68 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGtttt                                          >  1:784949/1‑68 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGttt                                           >  1:1408219/1‑67 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGttt                                           >  1:2879180/1‑67 (MQ=255)

                    aTCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCa                                        <  1:385478/70‑1 (MQ=255)

                       tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtct                                    >  1:2253968/1‑71 (MQ=255)

                       tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtct                                    >  1:1195992/1‑71 (MQ=255)

                       tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     >  1:1544829/1‑70 (MQ=255)

                       tttACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     >  1:2859744/1‑70 (MQ=255)

                       tttACGGCCAAAAATTTCATCGAAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGtt                                            >  1:1556580/1‑63 (MQ=255)

                         tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     <  1:2176211/68‑1 (MQ=255)

                         tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     <  1:2945981/68‑1 (MQ=255)

                         tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     <  1:3336798/68‑1 (MQ=255)

                         tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     <  1:2186045/68‑1 (MQ=255)

                         tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     <  1:2247806/68‑1 (MQ=255)

                         tACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAta                                     <  1:3895117/68‑2 (MQ=255)

                         tACGGCCAAAAATTTCATCGAAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAAtc                                     <  1:3757556/68‑1 (MQ=255)

                             gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:445299/1‑47 (MQ=255)

                             gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:931851/1‑47 (MQ=255)

                             gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:3657689/1‑47 (MQ=255)

                             gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:353967/1‑47 (MQ=255)

                             gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:288194/1‑47 (MQ=255)

                             gCCAAAAATTTCATCGTAGCCGACAATACGGAAGCGGTGAGTCACGc                                                      >  1:1898014/1‑47 (MQ=255)

                                         aTCGTAGCCGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCaaaa                   <  1:3900971/70‑1 (MQ=255)

                                                 cGACAATTCGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCTAAAAAGACTTTg          <  1:509876/71‑1 (MQ=255)

                                                 cGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTg          <  1:3877798/71‑1 (MQ=255)

                                                 cGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTg          <  1:3468438/71‑1 (MQ=255)

                                                 cGACAATACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTg          <  1:2717723/71‑1 (MQ=255)

                                                     aaTACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt      <  1:2945753/71‑1 (MQ=255)

                                                     aaTACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt      <  1:2573427/71‑1 (MQ=255)

                                                     aaTACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt      <  1:2570000/71‑1 (MQ=255)

                                                       tACGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCtt      <  1:1849428/69‑1 (MQ=255)

                                                         cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg  <  1:1075376/71‑1 (MQ=255)

                                                         cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg  <  1:914985/71‑1 (MQ=255)

                                                         cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg  <  1:1905205/71‑1 (MQ=255)

                                                         cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg  <  1:3155374/71‑1 (MQ=255)

                                                         cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg  <  1:3564438/71‑1 (MQ=255)

                                                         cGGAAGCGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCctg  <  1:3612716/71‑1 (MQ=255)

GGGGAATCGATAGAGATGTAATCTTTACGGCCAAAAATTTCATCGTAGCCGACAATACGGAAACGGTGAGTCACGCCATCGTCGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTG  >  W3110S.gb/4103239‑4103366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`