| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,154,666 | A→G | I23V (ATT→GTT) | yhfA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,154,666 | 0 | A | G | 96.5% | 198.1 / ‑1.8 | 58 | I23V (ATT→GTT) | yhfA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (30/26); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
CAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGGCGGCG > W3110S.gb/4154603‑4154731 | cAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa < 1:1276413/70‑1 (MQ=255)cAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa < 1:2715080/70‑1 (MQ=255) aaGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:139546/71‑1 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAgg > 1:2522612/1‑70 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:927262/1‑71 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGc > 1:382717/1‑71 (MQ=255) ggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTa > 1:3388780/1‑52 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:47374/1‑71 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:3563717/1‑71 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:2933593/1‑71 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:1739067/1‑71 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTAAGGCGATAAAg > 1:524716/1‑71 (MQ=255) gggTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:1125072/1‑70 (MQ=255) gggTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:676461/1‑70 (MQ=255) gggTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:3403807/1‑70 (MQ=255) gggTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:3132981/1‑70 (MQ=255) gggTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:1396772/1‑70 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTc > 1:773423/1‑56 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCg > 1:1508991/1‑61 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:2222323/1‑70 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAcc > 1:745917/1‑69 (MQ=255) ttCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTcc < 1:177629/70‑1 (MQ=255) ttCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTcc < 1:293097/70‑1 (MQ=255) tGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGAt < 1:1304830/69‑1 (MQ=255) tGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGAt < 1:3303307/69‑1 (MQ=255) tGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGAt < 1:1417397/69‑1 (MQ=255) tGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGAt < 1:2862733/69‑1 (MQ=255) tGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGAt < 1:1971987/69‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:639446/61‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:3704004/61‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1524182/61‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:3495933/71‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:4131676/71‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:665709/71‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:1846673/71‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:1082842/71‑1 (MQ=255) atcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg > 1:987772/1‑71 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:3185853/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:191456/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:2634234/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:1323736/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:2957627/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:1620700/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:3208246/56‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:274516/70‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGgcggcg < 1:814333/70‑1 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATgg < 1:540740/55‑1 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:3786501/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:4112369/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:1651760/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:3739105/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:490927/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:1708786/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:2690704/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:1165962/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:2259105/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:19672/1‑61 (MQ=255) caGGTTTTAATGGACGGCAACGCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGa > 1:541555/1‑61 (MQ=255) | CAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTTGATGGCGGCG > W3110S.gb/4154603‑4154731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |