| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,421,418 | C→T | R12Q (CGG→CAG) | yjfO ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,421,418 | 0 | C | T | 75.8% | 233.9 / 81.5 | 124 | R12Q (CGG→CAG) | yjfO | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base T (57/37); total (72/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTA > W3110S.gb/4421353‑4421466 | gctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGt > 1:600870/1‑69 (MQ=255)gctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTa > 1:1768417/1‑70 (MQ=255)gctgTGGCGTACCTTGCAGTGCGCTACAGGCAATTAACATCAACACCAATAATAAACTGGCAAACCGGTa > 1:2838372/1‑70 (MQ=255) tgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAAt < 1:4107466/69‑1 (MQ=255) gCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAAc > 1:2408376/1‑70 (MQ=255) gCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACg > 1:3163305/1‑71 (MQ=255) gCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACg > 1:2663826/1‑71 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:3499162/64‑1 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:3950957/64‑1 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:1898894/64‑1 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:1229226/64‑1 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:2763931/64‑1 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:3574999/64‑1 (MQ=255) aGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:1000537/64‑1 (MQ=255) tGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTa > 1:2229599/1‑64 (MQ=255) tGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTa > 1:3891598/1‑64 (MQ=255) tGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTa > 1:83289/1‑64 (MQ=255) ggcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTg > 1:1624113/2‑71 (MQ=255) cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCt > 1:3824555/1‑70 (MQ=255) cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTg > 1:3504233/1‑71 (MQ=255) cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTg > 1:3672001/1‑71 (MQ=255) cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTg > 1:556676/1‑71 (MQ=255) cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTg > 1:3376388/1‑71 (MQ=255) cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTg > 1:12992/1‑71 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:1635595/64‑1 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:1647211/64‑1 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:1385545/64‑1 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:2258200/64‑1 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:63352/64‑1 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:1446549/64‑1 (MQ=255) tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGttt < 1:2096956/64‑1 (MQ=255) aCTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt < 1:3985197/50‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCt < 1:3846070/47‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCt < 1:454199/47‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCt < 1:1338751/47‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCt < 1:1361014/47‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:3464706/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:3042185/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:2310645/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:1842646/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:133733/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:1276284/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:60034/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:1000790/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCT‑ACCAtagtg > 1:2762842/1‑71 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGc < 1:3644007/46‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGc < 1:2617361/46‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGc < 1:466959/46‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGc < 1:3293693/46‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGc < 1:1112184/46‑1 (MQ=255) cTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGc < 1:286965/46‑1 (MQ=255) cTTAACATCAACACAAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:976836/1‑71 (MQ=255) cTTAACAACAACACCAATAATAAACTGGCAAACTGCTAAATAACGCTATTACGTTTCCTGCTAACCAtagt > 1:2417281/1‑71 (MQ=255) tAACATCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTAt > 1:1161044/1‑47 (MQ=255) cATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGt < 1:3119142/49‑1 (MQ=255) cATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGt < 1:3766693/49‑1 (MQ=255) cATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGt < 1:3221478/49‑1 (MQ=255) cATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGt < 1:1226486/49‑1 (MQ=255) cATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGt < 1:357758/49‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:2949667/60‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:299547/60‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:1760065/60‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:1213459/60‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:1397065/60‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:1267986/60‑1 (MQ=255) tCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGttt > 1:1924853/1‑49 (MQ=255) tCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGttt > 1:356613/1‑49 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:133682/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:543794/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:2362042/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:2112767/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:1632822/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:1162680/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:3681240/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:868384/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:3160178/1‑60 (MQ=255) aaCACCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:3851941/1‑60 (MQ=255) cacCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta > 1:1284904/1‑71 (MQ=255) cacCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta > 1:3771018/1‑71 (MQ=255) cacCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta > 1:3811611/1‑71 (MQ=255) cacCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta > 1:2998958/1‑71 (MQ=255) cacCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta > 1:3650070/1‑71 (MQ=255) acCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTc > 1:353110/1‑45 (MQ=255) acCAATAATAAACTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCa < 1:2898391/55‑1 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:3857994/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:2047461/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:775609/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:2697767/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:2686810/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:3577742/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:1410119/1‑51 (MQ=255) aataaACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCAta > 1:2079503/1‑51 (MQ=255) aaCTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGc < 1:501577/56‑1 (MQ=255) aaCTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGc < 1:1225255/56‑1 (MQ=255) aaCTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGc < 1:4083199/56‑1 (MQ=255) aaCTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGc < 1:4015970/56‑1 (MQ=255) aCTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGc < 1:351471/55‑1 (MQ=255) cTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTaa > 1:229230/1‑40 (MQ=255) cTGGCAAACTGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCt > 1:2234299/1‑56 (MQ=255) cTGCCAAACTGGTAAATAACGCTATTACGTTTcctcct > 1:3270132/1‑38 (MQ=37) | GCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTA > W3110S.gb/4421353‑4421466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |