| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,553,062 | T→C | G32G (GGT→GGC) | fimG → | minor component of type 1 fimbriae |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,553,062 | 0 | T | C | 77.5% | 214.8 / 58.1 | 102 | G32G (GGT→GGC) | fimG | minor component of type 1 fimbriae |
| Reads supporting (aligned to +/- strand): ref base T (23/0); new base C (46/33); total (69/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGA > W3110S.gb/4552996‑4553121 | ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAgg > 1:1429606/1‑71 (MQ=255)ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAgg > 1:4046208/1‑71 (MQ=255)ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAgg > 1:3757778/1‑71 (MQ=255)ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAgg > 1:1094647/1‑71 (MQ=255)ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAgg > 1:1155682/1‑71 (MQ=255)ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAgg > 1:698398/1‑71 (MQ=255)ggcggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAgg > 1:4084960/1‑71 (MQ=255) ttGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCa > 1:1063189/1‑70 (MQ=255) ttGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCa > 1:2723267/1‑70 (MQ=255) ttGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCa > 1:52834/1‑70 (MQ=255) ttGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCa > 1:2722877/1‑70 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAATGTCGTCGCCAAACCgtg < 1:1096277/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:324970/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:922223/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:2385105/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:2344345/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:2336483/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:1916633/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:2019655/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:3420386/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:1605467/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:3590273/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:3990410/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:1388453/71‑1 (MQ=255) cTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCgtg < 1:544033/71‑1 (MQ=255) cAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:3912556/1‑71 (MQ=255) cAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:886638/1‑71 (MQ=255) cAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:2126979/1‑71 (MQ=255) cAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:2620454/1‑71 (MQ=255) cAGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:120749/1‑71 (MQ=255) aGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGtt < 1:3173000/59‑1 (MQ=255) aGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGtt < 1:2702708/59‑1 (MQ=255) aGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGtt < 1:273199/59‑1 (MQ=255) aGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGtt < 1:2746353/59‑1 (MQ=255) aGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGtt < 1:217523/59‑1 (MQ=255) aGGCAGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGtt < 1:227120/59‑1 (MQ=255) aGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:3610980/1‑66 (MQ=255) aGCCGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCaa > 1:376170/1‑66 (MQ=255) cGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:2557017/71‑1 (MQ=255) cGATGTCACCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:2802694/71‑1 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2830120/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:1419612/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2964405/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:3029712/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2706758/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:3093095/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2687792/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:4089153/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:392795/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:3890170/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:1424896/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2034687/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:250841/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2100882/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2166326/1‑56 (MQ=255) gATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:2240118/1‑56 (MQ=255) gATGTCACCATCACCGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:1946579/1‑56 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:1369193/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:2542669/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:3116107/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:1181384/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:2837027/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:2819753/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:2318079/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:3760892/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:3361568/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:1626846/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:654227/1‑71 (MQ=255) aCCATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAtct > 1:2696479/1‑71 (MQ=255) cATCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:1821004/1‑48 (MQ=255) cATCACGGTGAAAGGCAAGGTCGTCGCCAAACCGTGTACGGTTTccac > 1:1285414/1‑48 (MQ=39) tCACGGTGAACGGCAAGGTCGTCTCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:2895296/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:1736079/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:396601/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:101272/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:551387/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:2520130/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:1428562/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:1847148/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:879876/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg < 1:2026881/60‑1 (MQ=255) tCACGGTGAACGGCAAGGTCGTCGCCAAACCGTCTACGGTTTCCACCACCAATGCCACgg < 1:1543812/60‑1 (MQ=255) aCGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATGTCGGCGa > 1:101423/1‑71 (MQ=255) aCGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:1684154/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTcc > 1:3614407/1‑42 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:319674/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:2034659/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:210870/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:2188793/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:1742160/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:2540006/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:1319750/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:1658824/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:4073195/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:1379501/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:3266804/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:3430874/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:3495348/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGa > 1:3916450/1‑71 (MQ=255) aCGGTGAACGGCAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCAAGGTTGATCTCGGCGa > 1:250997/1‑71 (MQ=255) | GGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGA > W3110S.gb/4552996‑4553121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |