Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	644,447	C→T	G453S (GGC→AGC)	citT ←	citrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	644,447	0	C	T	100.0%	137.5 / NA	42	G453S (GGC→AGC)	citT	citrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/5);  total (37/5)

ACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCCACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCAT  >  W3110S.gb/644381‑644501                                                                   |                                                       aCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAg                                                    >  1:477969/1‑71 (MQ=255)         aTGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTaaa                                             >  1:3201095/1‑70 (MQ=255)         aTGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTaaa                                             >  1:2564535/1‑70 (MQ=255)            tAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAaataat                                         >  1:1515060/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:292170/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:844776/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:820413/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:802539/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:801765/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:252494/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:2418190/1‑71 (MQ=255)              aaTCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCa                                       >  1:1935594/1‑71 (MQ=255)                 cacaCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAcac                                    >  1:2348719/1‑69 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACTGCCGTAAATAATCAccc                                    >  1:802430/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:1955659/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:1093949/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:1163189/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:1567714/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:3651998/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:2174753/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:3124003/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:3104648/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:2859449/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:2209203/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:239813/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:2097095/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:2686553/1‑71 (MQ=255)                 cACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCAccc                                    >  1:1041798/1‑71 (MQ=255)                              gcgcCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTg                        <  1:352235/70‑1 (MQ=255)                              gcgcCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTg                        <  1:2051118/70‑1 (MQ=255)                              gcgcCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTg                        <  1:431233/70‑1 (MQ=255)                              gcgcCAAGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTg                        <  1:723296/70‑1 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACgg                 >  1:3238473/1‑71 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACgg                 >  1:3727813/1‑71 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACgg                 >  1:407352/1‑71 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACgg                 >  1:472853/1‑71 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACgg                 >  1:1784950/1‑71 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACgg                 >  1:2241959/1‑71 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACg                  >  1:56679/1‑70 (MQ=255)                                    aGACGCCAGTAATCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACg                  >  1:3127261/1‑70 (MQ=255)                                                tCTTTTGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACGGCGTCAGACAGcc     >  1:3174507/1‑71 (MQ=255)                                                    ttGATTTCACATAGCTACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCAt  <  1:2072645/70‑1 (MQ=255)                                                                   |                                                       ACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATAGCCACAGCCGTAAATAATCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCAT  >  W3110S.gb/644381‑644501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑