Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 904,996 G→A intergenic (‑107/‑19) ybjP ← / → ybjQ predicted lipoprotein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb904,9960GA95.1% 119.2 / ‑1.2 41intergenic (‑107/‑19)ybjP/ybjQpredicted lipoprotein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (16/23);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA  >  W3110S.gb/904932‑905062
                                                                |                                                                  
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:3882725/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:3877970/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:1704863/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:346203/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:2886764/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:1982178/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:2307828/71‑1 (MQ=255)
 aataatGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca                                                              <  1:2229329/70‑1 (MQ=255)
         tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:347231/71‑1 (MQ=255)
         tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:97870/71‑1 (MQ=255)
         tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:849182/71‑1 (MQ=255)
         tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:4092695/71‑1 (MQ=255)
         tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:3693982/71‑1 (MQ=255)
         tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:2447549/71‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:1338094/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:780072/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:625045/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:787502/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:3639222/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:1905605/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:2012626/57‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:595265/70‑1 (MQ=255)
          atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:971314/70‑1 (MQ=255)
           tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa                                                                  <  1:814526/56‑1 (MQ=255)
                          cGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTAc                                    <  1:50849/71‑1 (MQ=255)
                                aGTGTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc                               >  1:3898631/1‑70 (MQ=255)
                                aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc                               >  1:209417/1‑70 (MQ=255)
                                aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc                               >  1:2382143/1‑70 (MQ=255)
                                aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc                               >  1:3815196/1‑70 (MQ=255)
                                aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc                               >  1:3848837/1‑70 (MQ=255)
                                    tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa                                                 >  1:733226/1‑48 (MQ=255)
                                    tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa                                                 >  1:1567164/1‑48 (MQ=255)
                                    tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa                                                 >  1:859233/1‑48 (MQ=255)
                                    tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa                                                 >  1:1144402/1‑48 (MQ=255)
                                     gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:2171968/43‑1 (MQ=255)
                                     gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:1424697/43‑1 (MQ=255)
                                     gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:3500623/43‑1 (MQ=255)
                                     gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg                                                     <  1:337285/43‑1 (MQ=255)
                                       tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:931437/1‑43 (MQ=255)
                                       tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:1651789/1‑43 (MQ=255)
                                       tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:862547/1‑43 (MQ=255)
                                       tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:2949000/1‑43 (MQ=255)
                                       tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:1932483/1‑43 (MQ=255)
                                       tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:2790529/1‑43 (MQ=255)
                                       tAATGCTTTTGAGATCAAAGGCTTAACAAACAAGGAgatcgat                                                   >  1:1608577/1‑43 (MQ=38)
                                                   gaTCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc           <  1:3936015/71‑1 (MQ=255)
                                                   gaTCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc           <  1:1746137/71‑1 (MQ=255)
                                                             ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa  <  1:3654874/70‑1 (MQ=255)
                                                             ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa  <  1:2162458/70‑1 (MQ=255)
                                                                |                                                                  
TAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA  >  W3110S.gb/904932‑905062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: