Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 904,996 | G→A | intergenic (‑107/‑19) | ybjP ← / → ybjQ | predicted lipoprotein/conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 904,996 | 0 | G | A | 95.1% | 119.2 / ‑1.2 | 41 | intergenic (‑107/‑19) | ybjP/ybjQ | predicted lipoprotein/conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (16/23); total (16/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.12e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 |
TAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904932‑905062 | taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:3882725/71‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:3877970/71‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:1704863/71‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:346203/71‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2886764/71‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:1982178/71‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2307828/71‑1 (MQ=255) aataatGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2229329/70‑1 (MQ=255) tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:347231/71‑1 (MQ=255) tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:97870/71‑1 (MQ=255) tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:849182/71‑1 (MQ=255) tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:4092695/71‑1 (MQ=255) tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:3693982/71‑1 (MQ=255) tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:2447549/71‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:1338094/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:780072/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:625045/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:787502/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:3639222/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:1905605/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:2012626/57‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:595265/70‑1 (MQ=255) atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:971314/70‑1 (MQ=255) tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:814526/56‑1 (MQ=255) cGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTAc < 1:50849/71‑1 (MQ=255) aGTGTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:3898631/1‑70 (MQ=255) aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:209417/1‑70 (MQ=255) aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:2382143/1‑70 (MQ=255) aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:3815196/1‑70 (MQ=255) aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:3848837/1‑70 (MQ=255) tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:733226/1‑48 (MQ=255) tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:1567164/1‑48 (MQ=255) tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:859233/1‑48 (MQ=255) tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:1144402/1‑48 (MQ=255) gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:2171968/43‑1 (MQ=255) gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:1424697/43‑1 (MQ=255) gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:3500623/43‑1 (MQ=255) gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:337285/43‑1 (MQ=255) tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:931437/1‑43 (MQ=255) tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:1651789/1‑43 (MQ=255) tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:862547/1‑43 (MQ=255) tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:2949000/1‑43 (MQ=255) tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:1932483/1‑43 (MQ=255) tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:2790529/1‑43 (MQ=255) tAATGCTTTTGAGATCAAAGGCTTAACAAACAAGGAgatcgat > 1:1608577/1‑43 (MQ=38) gaTCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc < 1:3936015/71‑1 (MQ=255) gaTCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc < 1:1746137/71‑1 (MQ=255) ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:3654874/70‑1 (MQ=255) ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:2162458/70‑1 (MQ=255) | TAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904932‑905062 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |