| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 913,779 | A→G | L153P (CTG→CCG) | hcp ← | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 913,779 | 0 | A | G | 96.5% | 92.8 / ‑4.6 | 29 | L153P (CTG→CCG) | hcp | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (22/6); total (22/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTG > W3110S.gb/913722‑913846 | agaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCgag < 1:773645/71‑1 (MQ=255)agaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCgag < 1:182579/71‑1 (MQ=255)agaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCgag < 1:3762544/71‑1 (MQ=255) cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTc > 1:1659584/1‑70 (MQ=255) ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCgag < 1:4069099/57‑1 (MQ=255) ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCaa < 1:3761675/71‑1 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:920519/1‑56 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:681433/1‑56 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:1695804/1‑56 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:547984/1‑56 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:2411633/1‑56 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATcgccgc > 1:164178/1‑71 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATcgccgc > 1:241362/1‑71 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATcgccg > 1:1069608/1‑70 (MQ=255) aCCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:487261/71‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:3564688/58‑1 (MQ=255) aCAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtta > 1:891976/1‑69 (MQ=255) aCAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtta > 1:414336/1‑69 (MQ=255) taGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:2163925/2‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATc > 1:3654739/1‑40 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTa > 1:484067/1‑49 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtt > 1:1564284/1‑68 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:241804/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:1937511/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:199799/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:711387/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:3813507/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg > 1:1027075/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct > 1:201121/1‑70 (MQ=255) cagcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt < 1:2771457/42‑1 (MQ=255) agcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCt > 1:2450678/1‑70 (MQ=255) agcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCt > 1:2711273/1‑70 (MQ=255) agcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTg < 1:3858252/71‑1 (MQ=255) agcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTg < 1:1484259/71‑1 (MQ=255) agcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTg < 1:2463685/71‑1 (MQ=255) | AGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTG > W3110S.gb/913722‑913846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |