| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 53,012 | A→G | G135G (GGT→GGC) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 53,012 | 0 | A | G | 82.9% | 99.1 / 12.4 | 41 | G135G (GGT→GGC) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base G (12/22); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTG > W3110S.gb/52945‑53066 | gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg > 1:1510694/1‑71 (MQ=255)gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg > 1:3469808/1‑71 (MQ=255)gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg > 1:574255/1‑71 (MQ=255) gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAgg < 1:2575020/71‑1 (MQ=255) atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGc > 1:1316205/1‑71 (MQ=255) atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGc > 1:3338776/1‑71 (MQ=255) atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGc > 1:332799/1‑71 (MQ=255) atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGc > 1:1720054/1‑71 (MQ=255) atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGc > 1:2788315/1‑71 (MQ=255) tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcg < 1:2875525/71‑1 (MQ=255) atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtc > 1:555060/1‑71 (MQ=255) atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtc > 1:3667098/1‑71 (MQ=255) atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtc > 1:3516702/1‑71 (MQ=255) atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtc > 1:182037/1‑71 (MQ=255) atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgt > 1:453060/1‑70 (MQ=255) caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGaa > 1:3639563/1‑59 (MQ=255) caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGaa > 1:3696260/1‑59 (MQ=255) caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcgt > 1:1118418/1‑71 (MQ=255) caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcgt > 1:289582/1‑71 (MQ=255) accTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTtaat < 1:2345017/71‑1 (MQ=255) accTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTtaat < 1:2929903/71‑1 (MQ=255) cGAAAAACTCGGTATGGCCTGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:417207/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:1806652/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:1302359/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:1578038/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:1701252/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:1988281/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:2816604/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:2246751/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:2208630/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:2154580/68‑1 (MQ=255) cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:2146928/68‑1 (MQ=255) gAAAATCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt < 1:3626730/67‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:791421/70‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:972682/70‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:1127199/70‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:3211600/70‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:3072987/70‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:2342075/70‑1 (MQ=255) gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:1950557/70‑1 (MQ=255) gAAAAACTAGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg < 1:2826647/70‑1 (MQ=255) aCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa > 1:3995772/1‑40 (MQ=255) aCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa > 1:1851802/1‑40 (MQ=255) aCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa > 1:804334/1‑40 (MQ=255) aCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa > 1:96908/1‑40 (MQ=255) aCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa > 1:1704531/1‑40 (MQ=255) | GTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTG > W3110S.gb/52945‑53066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |