Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	927,929	C→T	Q563Q (CAG→CAA)	cydC ←	fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	927,929	0	C	T	100.0%	71.7 / NA	23	Q563Q (CAG→CAA)	cydC	fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/5);  total (18/5)

CAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCAT  >  W3110S.gb/927872‑927985                                                          |                                                         cAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAg                                               >  1:1735265/1‑69 (MQ=255) cAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAg                                               >  1:339710/1‑69 (MQ=255) cAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAg                                               >  1:2511142/1‑69 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:4075060/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:458392/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:4038634/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:513628/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:2456951/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:2406987/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:2334118/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:913975/1‑57 (MQ=255)               cAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCa                                             >  1:955251/1‑57 (MQ=255)                  taataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCt                            <  1:2525381/71‑1 (MQ=255)                  taataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCt                            <  1:761885/71‑1 (MQ=255)                  taataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCt                            <  1:2263599/71‑1 (MQ=255)                  taataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCt                            <  1:4015156/71‑1 (MQ=255)                  taataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCt                            <  1:1828922/71‑1 (MQ=255)                    ataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCtt                           >  1:2854354/1‑70 (MQ=255)                    ataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTg                          >  1:105134/1‑71 (MQ=255)                                                   aaCGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCaataat                 >  1:1029295/1‑49 (MQ=255)                                                   aaCGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCaataa                  >  1:4039531/1‑48 (MQ=255)                                                    aCGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGt         >  1:2858024/1‑56 (MQ=255)                                                    aCGCCCTTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCAt  >  1:1888625/1‑63 (MQ=255)                                                          |                                                         CAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCAT  >  W3110S.gb/927872‑927985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑