Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	941,935	C→T	T185T (ACC→ACT)	dmsA →	dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	941,935	0	C	T	100.0%	143.2 / NA	46	T185T (ACC→ACT)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/27);  total (19/27)

CTATCTGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGACTA  >  W3110S.gb/941869‑941991                                                                   |                                                         ctatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTgg                                                      >  1:2970758/1‑71 (MQ=255) ctatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTgg                                                      >  1:2353137/1‑71 (MQ=255) ctatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTgg                                                      >  1:1525560/1‑71 (MQ=255)  tatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGt                                                     >  1:912755/1‑71 (MQ=255)  tatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGt                                                     >  1:2342072/1‑71 (MQ=255)        aaCTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTcgcgcg                                               >  1:3887355/1‑71 (MQ=255)        aaCTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTcgcgcg                                               >  1:1171799/1‑71 (MQ=255)        aaCTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTcgcgcg                                               >  1:645431/1‑71 (MQ=255)             tGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtga                                          >  1:104098/1‑71 (MQ=255)             tGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtga                                          >  1:3548463/1‑71 (MQ=255)             tGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtga                                          >  1:1415134/1‑71 (MQ=255)             tGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtga                                          >  1:2215072/1‑71 (MQ=255)                cACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatga                                       <  1:1956849/71‑1 (MQ=255)                cACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatga                                       <  1:2493892/71‑1 (MQ=255)                cACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatga                                       <  1:3177105/71‑1 (MQ=255)                cACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatga                                       <  1:2897387/71‑1 (MQ=255)                cACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatga                                       <  1:3955174/71‑1 (MQ=255)                 aCCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGaa                                      >  1:3637413/1‑71 (MQ=255)                 aCCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGaa                                      >  1:3222540/1‑71 (MQ=255)                 aCCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGaa                                      >  1:1682379/1‑71 (MQ=255)                 aCCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGaa                                      >  1:1806596/1‑71 (MQ=255)                     gTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAActgc                                  >  1:89807/1‑71 (MQ=255)                     gTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAActgc                                  >  1:4055260/1‑71 (MQ=255)                     gTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAActg                                   >  1:3921862/1‑70 (MQ=255)                                    ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCt                        <  1:662107/66‑1 (MQ=255)                                    ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCt                        <  1:3782604/66‑1 (MQ=255)                                    ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCt                        <  1:256629/66‑1 (MQ=255)                                    ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCt                        <  1:249224/66‑1 (MQ=255)                                    ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCt                        <  1:1266067/66‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGTGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:2834638/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTTTCTGa                  <  1:2507716/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:76799/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:3020264/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:3463634/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:3315397/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:1593045/71‑1 (MQ=255)                                     cATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGa                  <  1:1663910/71‑1 (MQ=255)                                                    gccgccGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGac    <  1:2299589/70‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:3381686/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:327285/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:3151807/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:3121222/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:29726/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:2612657/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:2169301/71‑1 (MQ=255)                                                     ccgccgGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGacta  <  1:1741206/71‑1 (MQ=255)                                                                   |                                                         CTATCTGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGACTA  >  W3110S.gb/941869‑941991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑