Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	959,125	A→G	T351A (ACA→GCA)	serC →	3‑phosphoserine/phosphohydroxythreonine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	959,125	0	A	G	100.0%	18.9 / NA	7	T351A (ACA→GCA)	serC	3‑phosphoserine/phosphohydroxythreonine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/2);  total (5/2)

CCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAA  >  W3110S.gb/959096‑959181                              |                                                         ccATGCCGCTGGAAGGCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGc                 >  1:1277341/1‑71 (MQ=255) ccATGCCGCTGGAAGGCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGc                 >  1:1463624/1‑71 (MQ=255) ccATGCCGCTGGAAGGCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGc                 >  1:976285/1‑71 (MQ=255) ccATGCCGCTGGAAGGCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACAGTTAATGc                 >  1:2842116/1‑71 (MQ=255)      ccGCTGGAAGGCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGaaa            >  1:2391517/1‑71 (MQ=255)                gCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTaa  <  1:2120276/71‑1 (MQ=255)                gCGTTAAAGCGCTGGCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTaa  <  1:347822/71‑1 (MQ=255)                              |                                                         CCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAA  >  W3110S.gb/959096‑959181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑