| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,016,291 | T→C | V52A (GTA→GCA) | rmf → | ribosome modulation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,016,291 | 0 | T | C | 97.8% | 152.6 / ‑4.0 | 45 | V52A (GTA→GCA) | rmf | ribosome modulation factor |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (9/35); total (9/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAC > W3110S.gb/1016225‑1016358 | ccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcagt < 1:1410416/70‑1 (MQ=255) tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATg < 1:582678/70‑1 (MQ=255) tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATg < 1:3037767/70‑1 (MQ=255) tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATg < 1:751013/70‑1 (MQ=255) tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATg < 1:1692435/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGTCGGACAGGGCAGTAATGGc < 1:1281947/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGcc > 1:2011914/1‑71 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGcc > 1:3344042/1‑71 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGcc > 1:2300533/1‑71 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGcc > 1:3946496/1‑71 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:4100332/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:161773/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:2129520/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:847100/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:2732500/70‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:2877650/70‑1 (MQ=255) cGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGa > 1:3922083/1‑71 (MQ=255) cGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGa > 1:2244620/1‑71 (MQ=255) cGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGa > 1:3722128/1‑71 (MQ=255) cGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGa > 1:4728/1‑71 (MQ=255) cGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGa > 1:1064171/1‑71 (MQ=255) aTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGG‑GGACAGGGCAGTAATGGCCTGATTCTGtc < 1:509466/71‑1 (MQ=255) tCAAAGGTCACATTGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:569403/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:2752288/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:3921722/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:3692132/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:3348346/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:3212526/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:2924354/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:2439119/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:2003253/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:1617915/71‑1 (MQ=255) tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGtc < 1:887690/71‑1 (MQ=255) gTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:553113/54‑1 (MQ=255) gggAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGc < 1:1997710/42‑1 (MQ=255) gagaAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa < 1:2818477/71‑1 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtt < 1:916152/71‑1 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtt < 1:878501/71‑1 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtt < 1:3681120/71‑1 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtt < 1:145625/71‑1 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtt < 1:11642/71‑1 (MQ=255) aCAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCTCCTTTTGa < 1:1450975/71‑1 (MQ=255) aCAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGa < 1:3792775/71‑1 (MQ=255) aCAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGa < 1:750244/71‑1 (MQ=255) aCAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGa < 1:1390689/71‑1 (MQ=255) gggCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAc < 1:1152408/71‑1 (MQ=255) | CCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAC > W3110S.gb/1016225‑1016358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |