Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,020,867	A→G	S159P (TCC→CCC)	sulA ←	SOS cell division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,020,867	0	A	G	100.0%	55.0 / NA	16	S159P (TCC→CCC)	sulA	SOS cell division inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/11);  total (5/11)

GATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAC  >  W3110S.gb/1020814‑1020929                                                      |                                                               gATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGt                                               <  1:1262429/71‑1 (MQ=255) gATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGt                                               <  1:2737707/71‑1 (MQ=255) gATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGt                                               <  1:470215/71‑1 (MQ=255)                    tAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGtc                                                        >  1:2255488/1‑43 (MQ=255)                    tAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGtc                                                        >  1:3446817/1‑43 (MQ=255)                            cAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataa                    >  1:683141/1‑71 (MQ=255)                            cAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataa                    >  1:877918/1‑71 (MQ=255)                                ttAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATaaa                <  1:3047484/71‑1 (MQ=255)                                      tGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCAt          <  1:42234/71‑1 (MQ=255)                                           ttttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt     >  1:2433925/1‑71 (MQ=255)                                               aGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAc  <  1:1068117/70‑1 (MQ=255)                                               aGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAc  <  1:2101439/70‑1 (MQ=255)                                               aGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAc  <  1:2120329/70‑1 (MQ=255)                                               aGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAc  <  1:4026106/70‑1 (MQ=255)                                               aGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCCCTTACCGGACGCATAATAAACCCCATAGCGTTAc  <  1:3807758/70‑1 (MQ=255)                                                gCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAc  <  1:479411/69‑1 (MQ=255)                                                      |                                                               GATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTAC  >  W3110S.gb/1020814‑1020929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑