Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,056,565	G→A	G12G (GGC→GGT)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,056,565	0	G	A	100.0%	132.3 / NA	44	G12G (GGC→GGT)	torS	hybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/24);  total (20/24)

ATAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAG  >  W3110S.gb/1056500‑1056631                                                                  |                                                                   aTAAAGCGCAGGTTGTACCATCCCACCAGTGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCa                                                                <  1:3074139/70‑1 (MQ=255) aTAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCa                                                                <  1:2008969/70‑1 (MQ=255) aTAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCa                                                                <  1:3537346/70‑1 (MQ=255) aTAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCa                                                                <  1:572782/70‑1 (MQ=255) aTAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCa                                                                <  1:2943655/70‑1 (MQ=255)      gcgcAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATcc                                                             >  1:2870171/1‑68 (MQ=255)      gcgcAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCag                                                           >  1:343320/1‑70 (MQ=255)      gcgcAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCa                                                            >  1:883654/1‑69 (MQ=255)      gcgcAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCa                                                            >  1:1508846/1‑69 (MQ=255)      gcgcAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCa                                                            >  1:3609898/1‑69 (MQ=255)      gcgcAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCa                                                            >  1:665666/1‑69 (MQ=255)                  ccATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGt                                                >  1:702163/1‑69 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAggg                                           >  1:2293474/1‑69 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGt                                          >  1:575290/1‑70 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGt                                          >  1:4043847/1‑70 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGt                                          >  1:3675071/1‑70 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGt                                          >  1:692497/1‑70 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGt                                          >  1:2170245/1‑70 (MQ=255)                       ccaccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTAAGGGt                                          >  1:3835016/1‑70 (MQ=255)                       ccaccaGGCTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTAGGGta                                               >  1:3899968/1‑64 (MQ=255)                            aGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAAtt                                    <  1:3069377/71‑1 (MQ=255)                            aGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAAtt                                    <  1:1912096/71‑1 (MQ=255)                            aGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAAtt                                    <  1:3780455/71‑1 (MQ=255)                            aGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAAtt                                    <  1:3807712/71‑1 (MQ=255)                                    ggTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcggtcg                            >  1:119691/1‑71 (MQ=255)                                    ggTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcggtcg                            >  1:1352466/1‑71 (MQ=255)                                    ggTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcggtcg                            >  1:124483/1‑71 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:345893/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:600882/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:507759/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:4079420/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:3912123/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:3416715/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:3305896/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:3114235/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:2684157/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:2635853/43‑1 (MQ=255)                                          ggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCgg                                                  <  1:1422771/43‑1 (MQ=255)                                           gTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACt                     >  1:3066202/1‑71 (MQ=255)                                           gTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACt                     >  1:658960/1‑71 (MQ=255)                                               aCAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTa                  <  1:2638583/70‑1 (MQ=255)                                               aCAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTa                  <  1:1397039/70‑1 (MQ=255)                                               aCAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTa                  <  1:953052/70‑1 (MQ=255)                                                           gggCAACACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGaaaaa         <  1:4085141/67‑1 (MQ=255)                                                               aaaaCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAg  <  1:4073193/70‑1 (MQ=255)                                                                  |                                                                   ATAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAG  >  W3110S.gb/1056500‑1056631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑